Reference - Detail
| RRC ID | 29854 |
|---|---|
| Author | Sullivan MA, Cannon JF, Webb FH, Bock RM. |
| Title | Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine. |
| Journal | J Bacteriol |
| Abstract |
Mutations in three Escherichia coli K-12 genes were isolated that reduce the efficiency of the lysine-inserting nonsense suppressor supL. These antisuppressor mutations asuD, asuE, and asuF map at 61.9, 25.3, and 76.3 min, respectively, on the E. coli chromosome. Biochemical and genetic analysis of the mutant strains revealed the reason for the antisuppressor phenotype for two of these genes. The activity of lysyl-tRNA synthetase was reduced in strains with asuD mutations. The modification of 5-methylaminomethyl-2-thiouridine, the wobble base of tRNALys, was impaired in asuE mutant strains, presumably at the 2-thiolation step. |
| Volume | 161(1) |
| Pages | 368-76 |
| Published | 1985-1-1 |
| DOI | 10.1128/jb.161.1.368-376.1985 |
| PMID | 3881393 |
| PMC | PMC214881 |
| MeSH | Chromosome Mapping Chromosomes, Bacterial Escherichia coli / genetics* Lysine-tRNA Ligase / metabolism Plasmids RNA, Transfer, Amino Acyl / metabolism Suppression, Genetic* Thiouridine / analogs & derivatives* Thiouridine / genetics Transduction, Genetic |
| IF | 3.006 |
| Times Cited | 76 |
| WOS Category | MICROBIOLOGY |
| Resource | |
| Prokaryotes E. coli | ME8386 ME8387 ME8388 ME8389 ME8390 ME8391 |