Reference - Detail
| RRC ID | 29876 |
|---|---|
| Author | Thomas M, Cameron JR, Davis RW. |
| Title | Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
A bacteriophage lambda strain has been constructed and a method developed by which DNA from potentially any source can be covalently inserted through EcoRI cohesive ends into the middle of the lambda DNA. These hybrid DNAs can infect nonrestricting Escherichia coli cells and can then propagate as plaque-forming phage. A unique feature of this lambda strain is that extra DNA in the middle of its genome is required for plaque formation. A large number of such phages have been produced with E. coli DNA and Drosophila melanogaster DNA. |
| Volume | 71(11) |
| Pages | 4579-83 |
| Published | 1974-11-1 |
| DOI | 10.1073/pnas.71.11.4579 |
| PMID | 4216019 |
| PMC | PMC433931 |
| MeSH | Animals Chromosome Mapping Coliphages* / growth & development DNA* DNA, Viral* Drosophila melanogaster Electrophoresis, Starch Gel Endonucleases Escherichia coli Hybridization, Genetic Hydrolysis Lysogeny Microscopy, Electron Nucleic Acid Hybridization Recombination, Genetic* Viral Plaque Assay Virus Replication* |
| IF | 9.412 |
| Resource | |
| Prokaryotes E. coli | ME6224 |