論文 - 詳細
| RRC ID | 30032 |
|---|---|
| 著者 | Spratt SK, Black PN, Ragozzino MM, Nunn WD. |
| タイトル | Cloning, mapping, and expression of genes involved in the fatty acid-degradative multienzyme complex of Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
Two protein subunits (42,000 and 78,000 daltons) encoded by the fadAB genes form a multifunctional enzyme complex containing thiolase, 3-hydroxyacyl-coenzyme A dehydrogenase, crotonase , epimerase, and isomerase activities (S. Pawar and H. Schulz, J. Biol. Chem. 256:3894-3899, 1981). In an attempt to characterize the structural organization and regulatory properties of these genes, a 5.2-kilobase pair fragment containing the fadAB genes has been isolated. Plasmids containing this fragment (i) complement mutations in the fadAB genes; (ii) overproduce by 10- to 50-fold thiolase, 3-hydroxyacyl-coenzyme A dehydrogenase and crotonase ; and (iii) specify a 42,000- and a 78,000-dalton protein. The fadA gene, which encodes the 42,000-dalton protein, has been localized within the original clone to a 3.3-kilobase pair fragment. Thiolase activity, which is encoded by the 42,000-dalton protein, was not observed in the absence of the 78,000-dalton protein, suggesting that an intact complex is required for function. Transposon Tn5 insertional mutagenesis of the cloned fadAB genes has demonstrated that both fadA and fadB are transcribed as a single transcriptional unit with the direction of transcription from fadA to fadB . The molecular cloning and characterization of the fadAB region confirm the original genetic contention that the genes encoding the proteins for the multifunctional complex form an operon. |
| 巻・号 | 158(2) |
| ページ | 535-42 |
| 公開日 | 1984-5-1 |
| DOI | 10.1128/jb.158.2.535-542.1984 |
| PMID | 6144665 |
| PMC | PMC215462 |
| MeSH | 3-Hydroxyacyl CoA Dehydrogenases / genetics Acetyl-CoA C-Acetyltransferase / genetics Chromosome Mapping Chromosomes, Bacterial Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Enoyl-CoA Hydratase / genetics Escherichia coli / enzymology Escherichia coli / genetics* Fatty Acids / metabolism* Genes, Bacterial* Genetic Complementation Test Multienzyme Complexes / genetics* Operon* Transcription, Genetic |
| IF | 3.006 |
| 引用数 | 35 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME8397 ME8398 |