| RRC ID |
30038
|
| Author |
Sadler JR, Tecklenburg M, Betz JL.
|
| Title |
Plasmids containing many tandem copies of a synthetic lactose operator.
|
| Journal |
Gene
|
| Abstract |
Up to 12 tandem copies of the lactose operator sequence AATTCCACATGTGGAATTGTGAGCGGATAACAATTTGTGG (3') GGTGTACACCTTAACACTCGCCTATTGTTAAACACCTTAA (5') have been cloned in the EcoRI site of plasmid pMB9. A 12-operator plasmid is about 8% operator by weight and represents a rich source of this DNA segment. A procedure for the rapid and convenient isolation of operator in mg quantities is presented. The lifetimes of complexes formed between repressor and oligo-operator plasmids increased with increasing numbers of tandem operators per plasmid. Evidence is presented indicating that only one tetrameric repressor molecule binds strongly to a segment of four (or fewer) tandem operators, but that two repressor molecules can be accommodated on segments containing at least six tandem operators.
|
| Volume |
8(3)
|
| Pages |
279-300
|
| Published |
1980-2-1
|
| DOI |
10.1016/0378-1119(80)90005-0
|
| PII |
0378-1119(80)90005-0
|
| PMID |
6244215
|
| MeSH |
Base Sequence
DNA Restriction Enzymes / metabolism
DNA, Recombinant / analysis*
Electrophoresis
Escherichia coli / genetics
Lac Operon*
Plasmids*
|
| IF |
2.984
|
| Times Cited |
150
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Prokaryotes E. coli |
ME8134
ME8135
ME8136
ME8133 |
