Reference - Detail
| RRC ID | 30071 |
|---|---|
| Author | Burns DM, Beacham IR. |
| Title | Positive selection vectors: a small plasmid vector useful for the direct selection of Sau3A-generated overlapping DNA fragments. |
| Journal | Gene |
| Abstract |
A positive selection plasmid vector ( pLA7 ), containing a unique BclI site, and its use in the facile selection of a library of overlapping DNA fragments, generated by a partial digest with Sau3A, is described. Selection depends on the 5-fluorouracil + 5' AMP resistance of upp- ush - [ pLA7ush ::cloned DNA] cells, whereas upp- ush - [ pLA7ush +] cells are sensitive under the same conditions. |
| Volume | 27(3) |
| Pages | 323-5 |
| Published | 1984-3-1 |
| DOI | 10.1016/0378-1119(84)90077-5 |
| PII | 0378-1119(84)90077-5 |
| PMID | 6329908 |
| MeSH | Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes* Deoxyribonucleases, Type II Site-Specific* Genetic Vectors* Nucleotidases / genetics Plasmids* Selection, Genetic |
| IF | 2.984 |
| Times Cited | 18 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | ME8614 pLA7 |