論文 - 詳細
| RRC ID | 30081 |
|---|---|
| 著者 | Hanahan D. |
| タイトル | Studies on transformation of Escherichia coli with plasmids. |
| ジャーナル | J Mol Biol |
| Abstract |
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth in medium containing elevated levels of Mg2+, and incubation of the cells at 0 degrees C in a solution of Mn2+, Ca2+, Rb+ or K+, dimethyl sulfoxide, dithiothreitol, and hexamine cobalt (III). Transformation efficiency declines linearly with increasing plasmid size. Relaxed and supercoiled plasmids transform with similar probabilities. Non-transforming DNAs compete consistent with mass. No significant variation is observed between competing DNAs of different source, complexity, length or form. Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmids. |
| 巻・号 | 166(4) |
| ページ | 557-80 |
| 公開日 | 1983-6-5 |
| DOI | 10.1016/s0022-2836(83)80284-8 |
| PII | S0022-2836(83)80284-8 |
| PMID | 6345791 |
| MeSH | Cations DNA, Superhelical Dimethyl Sulfoxide / pharmacology Dithiothreitol / pharmacology Escherichia coli / drug effects Escherichia coli / genetics* Escherichia coli / growth & development Plasmids* / drug effects Species Specificity Transformation, Bacterial* / drug effects |
| IF | 4.76 |
| 引用数 | 9216 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1019 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.2 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9033 |