RRC ID 30084
Author Young KK, Edlin G.
Title Physical and genetical analysis of bacteriophage T4 generalized transduction.
Journal Mol Gen Genet
Abstract This report describes a comparison of the efficiency of transduction of genes in E. coli by the generalized transducing bacteriophages T4GT7 and P1CM. Both phages are capable of transducing many genetic markers in E. coli although the frequency of transduction for particular genes varies over a wide range. The frequency of transduction for most genes depends on which transducing phage is used as well as on the donor and recipient bacterial strains. Analysis of T4GT7 phage lysates by cesium chloride density gradient centrifugation shows that transducing phage particles contain primarily bacterial DNA and carry little, if any, phage DNA. In this regard transducing phages P1CM and T4GT7 are similar; both phages package either bacterial or phage DNA but not both DNAs into the same particle.
Volume 192(1-2)
Pages 241-6
Published 1983-1-1
DOI 10.1007/BF00327673
PMID 6358795
MeSH Coliphages / genetics DNA, Bacterial / genetics Escherichia coli / genetics* Genes, Bacterial T-Phages / genetics* Transduction, Genetic*
Times Cited 30
Prokaryotes E. coli ME6040