論文 - 詳細
| RRC ID | 3010 |
|---|---|
| 著者 | Ohori A, Endo S, Sano A, Yokoyama K, Yarita K, Yamaguchi M, Kamei K, Miyaji M, Nishimura K. |
| タイトル | Rapid identification of Ochroconis gallopava by a loop-mediated isothermal amplification (LAMP) method. |
| ジャーナル | Vet Microbiol |
| Abstract |
Ochroconis gallopava is a species of dematiaceous fungi recognized as a causative agent of zoonotic and emerging fungal infections. It affects the central nervous system and respiratory tracts of humans, birds and cats. We designed O. gallopava species-specific primer sets to aid in its identification by a loop-mediated isothermal amplification (LAMP) method based on the D1/D2 domain of the LSU rDNA sequence. The LAMP method successfully detected the gene from both fungal DNA and experimentally infected brains and spleens of mice and will be helpful in the diagnosis of O. gallopava infection. |
| 巻・号 | 114(3-4) |
| ページ | 359-65 |
| 公開日 | 2006-5-31 |
| DOI | 10.1016/j.vetmic.2005.11.064 |
| PII | S0378-1135(05)00453-0 |
| PMID | 16386386 |
| MeSH | Animals Arthrodermataceae / genetics Arthrodermataceae / isolation & purification* Base Sequence DNA, Fungal / analysis* Dermatomycoses / diagnosis Dermatomycoses / microbiology Dermatomycoses / veterinary* Mice Molecular Sequence Data Nucleic Acid Amplification Techniques / methods Nucleic Acid Amplification Techniques / veterinary* Polymerase Chain Reaction Sensitivity and Specificity Species Specificity |
| IF | 3.03 |
| 引用数 | 27 |
| WOS 分野 | VETERINARY SCIENCES MICROBIOLOGY |
| リソース情報 | |
| 病原微生物 | IFM 4808 IFM 4827 IFM 4957 IFM 4966 IFM 5740 IFM 5830 IFM 40821 IFM 41316 IFM 41329 IFM 41459 IFM 41460 IFM 41473 IFM 41474 IFM 41597 IFM 41708 IFM 47068 IFM 50993 IFM 52187 IFM 52602 IFM 52603 IFM 52604 IFM 52605 IFM 52653 IFM 52654 IFM 52655 IFM 52656 IFM 52657 IFM 52658 IFM 52663 IFM 52664 IFM 52846 IFM 52847 IFM 52848 IFM 52849 IFM 52850 IFM 52851 IFM 52852 IFM 52853 IFM 52854 IFM 52855 IFM 52856 IFM 52857 IFM 52880 IFM 52974 IFM 52975 IFM 52976 IFM 52977 IFM 4808 IFM 4827 IFM 4957 IFM 4966 IFM 5740 O. gallopava? |