RRC ID 30103
Author Jagura-Burdzy G, Hulanicka D.
Title Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon.
Journal J Bacteriol
Abstract Strains of escherichia coli were constructed in which the lacZ gene is fused to cysB, the positive regulator gene of the cysteine regulon. The fusion strains were used to study the regulation of the cysB gene by assaying the fused lacZ gene product. The introduction of a cysB allele, either on a plasmid or on an episome to the fusion strains, resulted in the decrease of beta-galactosidase activity. This implies that the cysB gene expression is autoregulated by its own product. The direction of cysB gene transcription was determined to be clockwise.
Volume 147(3)
Pages 744-51
Published 1981-9-1
DOI 10.1128/jb.147.3.744-751.1981
PMID 6792186
PMC PMC216109
MeSH Cysteine / biosynthesis* DNA, Recombinant Escherichia coli / genetics Gene Expression Regulation* Genes, Bacterial* Genes, Regulator* Operon* Transcription, Genetic beta-Galactosidase / biosynthesis
IF 3.006
Times Cited 42
Prokaryotes E. coli ME8284