RRC ID 30107
Author Cooper RA, Skinner MA.
Title Catabolism of 3- and 4-hydroxyphenylacetate by the 3,4-dihydroxyphenylacetate pathway in Escherichia coli.
Journal J. Bacteriol.
Abstract Various strains of Escherichia coli (but not strain K-12) were found to grow on 3-hydroxyphenylacetate and 4-hydroxyphenylacetate. Both compounds were catabolized by the same pathway, with 3,4-dihydroxyphenylacetate as a substrate for fission of the benzene nucleus, and with pyruvate and succinate as products. All the necessay enzymes were demonstrated in cell extracts prepared from induced cells but were essentially absent from uninduced cells. Mutants unable to grow on 3- and 4-hydroxyphenylactetate were defective in particular enzymes of the pathway. The characteristics of certain mutants indicated that either uptake or hydroxylation of 3- and 4-hydroxyphenylacetate may involve a common protein component. E. coli also grew on 3,4-hydroxyphenylacetate, with induction of the enzyme necessary for its degradation but not those for the uptake-hydroxylation of 3- and 4-hydroxyphenylacetate.
Volume 143(1)
Pages 302-6
Published 1980-7
PMID 6995433
PMC PMC294232
MeSH 3,4-Dihydroxyphenylacetic Acid / metabolism Aldehyde Oxidoreductases / metabolism Dioxygenases* Escherichia coli / growth & development Escherichia coli / metabolism* Mixed Function Oxygenases / metabolism Oxygenases / metabolism Phenylacetates / metabolism* Pyruvates / biosynthesis Succinate-Semialdehyde Dehydrogenase Succinates / metabolism
IF 3.234
Times Cited 98
Prokaryotes E. coli ME8605