RRC ID 3012
著者 Ono T, Park KS, Ueta M, Iida T, Honda T.
タイトル Identification of proteins secreted via Vibrio parahaemolyticus type III secretion system 1.
ジャーナル Infect Immun
Abstract Vibrio parahaemolyticus, a gram-negative marine bacterium, is an important pathogen causing food-borne gastroenteritis or septicemia. Recent genome sequencing of the RIMD2210633 strain (a Kanagawa phenomenon-positive clinical isolate of serotype O3:K6) revealed that the strain has two sets of gene clusters that encode the type III secretion system (TTSS) apparatus. The first cluster, TTSS1, is located on the large chromosome, and the second, TTSS2, is on the small chromosome. Previously, we reported that TTSS1 is involved in the cytotoxicity of the RIMD2210633 strain against HeLa cells. Here, we analyzed proteins secreted via the TTSS apparatus encoded by TTSS1 by using two-dimensional gel electrophoresis and identified the proteins encoded by genes VP1680, VP1686, and VPA450. To investigate the roles of those secreted proteins, we constructed and analyzed a series of deletion mutants. Flow cytometry analysis using fluorescence-activated cell sorting with fluorescein isothiocyanate-labeled annexin V demonstrated that the TTSS1-dependent cell death was by apoptosis. The cytotoxicity to HeLa cells was related to one of the newly identified secreted proteins encoded by VP1680. Adenylate cyclase fusion protein studies proved that the newly identified secreted proteins were translocated into HeLa cells. Thus, these appear to be the TTSS effector proteins in V. parahaemolyticus.
巻・号 74(2)
ページ 1032-42
公開日 2006-2-1
DOI 10.1128/IAI.74.2.1032-1042.2006
PII 74/2/1032
PMID 16428750
PMC PMC1360304
MeSH Amino Acid Sequence Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism* Electrophoresis, Gel, Two-Dimensional Gene Deletion Gene Expression Regulation, Bacterial* HeLa Cells Humans Molecular Sequence Data Vibrio parahaemolyticus / genetics Vibrio parahaemolyticus / metabolism Vibrio parahaemolyticus / pathogenicity*
IF 3.201
引用数 107
WOS 分野 INFECTIOUS DISEASES IMMUNOLOGY
リソース情報
病原微生物 RIMD 2210633 RIMD 2212472?