| RRC ID |
30146
|
| Author |
Bates DB, Boye E, Asai T, Kogoma T.
|
| Title |
The absence of effect of gid or mioC transcription on the initiation of chromosomal replication in Escherichia coli.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Despite the widely accepted view that transcription of gid and mioC is required for efficient initiation of cloned oriC, we show that these transcriptions have very little effect on initiation of chromosome replication at wild-type chromosomal oriC. Furthermore, neither gid nor mioC transcription is required in cells deficient in the histone-like proteins Fis or IHF. However, oriC that is sufficiently impaired for initiation by deletion of DnaA box R4 requires transcription of at least one of these genes. We conclude that transcription of mioC and especially gid is needed to activate oriC only under suboptimal conditions. We suggest that either the rifampicin-sensitive step of initiation is some other transcription occurring from promoter(s) within oriC, or the original inference of transcriptional activation derived from the rifampicin experiments is incorrect.
|
| Volume |
94(23)
|
| Pages |
12497-502
|
| Published |
1997-11-11
|
| DOI |
10.1073/pnas.94.23.12497
|
| PMID |
9356478
|
| PMC |
PMC25015
|
| MeSH |
Bacterial Proteins / genetics*
Base Sequence
Chromosomes, Bacterial / genetics*
DNA Replication*
DNA, Bacterial / genetics
Escherichia coli / genetics*
Escherichia coli Proteins*
Flavoproteins*
Genes, Bacterial*
Molecular Sequence Data
Plasmids
Transcription, Genetic
|
| IF |
9.412
|
| Times Cited |
33
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
ME9391
ME9552 |
