RRC ID 30161
著者 Yoshida KI, Ishio I, Nagakawa E, Yamamoto Y, Yamamoto M, Fujita Y.
タイトル Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
ジャーナル Microbiology (Reading)
Abstract Within the framework of the international project 'The functional analysis of the Bacillus subtilis genome' in Japan and Europe, the gene expression and transcription organization of the gntZ-ywaA region (160 kb) of the B. subtilis genome has been systematically analysed. First, all unanalysed genes comprising more than 80 amino acids (125 genes) in this region were inactivated through integration of plasmid pMUTIN. No essential gene was found which could not be inactivated. All the integrants grew normally in both nutrient sporulation medium and glucose minimal medium. But an integrant in the yxbG gene exhibited an oligosporogenic phenotype in the nutrient sporulation medium. The synthesis of beta-galactosidase was examined, as a reporter for expression of the inactivated genes, during growth and sporulation in the two media. The results indicated that 36% of the promoters were inactive when cells were grown in at least one of these two media. Furthermore, the transcription of the 119 genes in this region was analysed by Northern blotting, resulting in a transcription map. The results indicate that the gntZ-ywaA region contains at least 24 polycistronic operons, including several published ones. The operons newly found in this work are yxaAB, yxaGH, yxaJKL, yxbBA-yxnB-asnH-yxaM, yxbCD, yxcED, yxdJK, yxeFGH, yxeKLMNOPQ, yxeR-yxxB, hutPHUIGM, bgIPH-yxiE, wapA-yxxG, yxiM-deaD, katB-yxiS, yxjCDEF, yxjJI and yxkF-mmsX.
巻・号 146 ( Pt 3)
ページ 573-579
公開日 2000-3-1
DOI 10.1099/00221287-146-3-573
PMID 10746760
MeSH Bacillus subtilis / genetics* Bacillus subtilis / growth & development Bacterial Proteins / genetics Gene Expression* Genome, Bacterial* Mutagenesis, Insertional Plasmids / genetics Transcription, Genetic* beta-Galactosidase / biosynthesis beta-Galactosidase / genetics
IF 2.138
引用数 58
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME9645