RRC ID 30185
著者 Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y.
タイトル myo-Inositol catabolism in Bacillus subtilis.
ジャーナル J Biol Chem
Abstract The iolABCDEFGHIJ operon of Bacillus subtilis is responsible for myo-inositol catabolism involving multiple and stepwise reactions. Previous studies demonstrated that IolG and IolE are the enzymes for the first and second reactions, namely dehydrogenation of myo-inositol to give 2-keto-myo-inositol and the subsequent dehydration to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione. In the present studies the third reaction was shown to be the hydrolysis of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione catalyzed by IolD to yield 5-deoxy-d-glucuronic acid. The fourth reaction was the isomerization of 5-deoxy-D-glucuronic acid by IolB to produce 2-deoxy-5-keto-D-gluconic acid. Next, in the fifth reaction 2-deoxy-5-keto-D-gluconic acid was phosphorylated by IolC kinase to yield 2-deoxy-5-keto-D-gluconic acid 6-phosphate. IolR is known as the repressor controlling transcription of the iol operon. In this reaction 2-deoxy-5-keto-D-gluconic acid 6-phosphate appeared to be the intermediate acting as inducer by antagonizing DNA binding of IolR. Finally, IolJ turned out to be the specific aldolase for the sixth reaction, the cleavage of 2-deoxy-5-keto-D-gluconic acid 6-phosphate into dihydroxyacetone phosphate and malonic semialdehyde. The former is a known glycolytic intermediate, and the latter was previously shown to be converted to acetyl-CoA and CO(2) by a reaction catalyzed by IolA. The net result of the inositol catabolic pathway in B. subtilis is, thus, the conversion of myo-inositol to an equimolar mixture of dihydroxyacetone phosphate, acetyl-CoA, and CO(2).
巻・号 283(16)
ページ 10415-24
公開日 2008-4-18
DOI 10.1074/jbc.M708043200
PII S0021-9258(20)62008-7
PMID 18310071
MeSH Aldehyde-Lyases / metabolism Bacillus subtilis / metabolism* Bacterial Proteins / metabolism Carbon-Carbon Lyases / physiology Catalysis DNA / metabolism DNA-Binding Proteins / metabolism Dihydroxyacetone Phosphate / metabolism Fructose-Bisphosphate Aldolase / physiology Gene Expression Regulation, Bacterial* Gluconates / metabolism Glucuronic Acid / metabolism Hydrolases / physiology Hydrolysis Inositol / metabolism* Models, Biological Models, Chemical Phosphotransferases / physiology Protein Binding Repressor Proteins / metabolism
IF 4.238
引用数 70
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME9655 ME9656 ME9664
原核生物(枯草菌) MBS169 MBS170 MBS171