論文 - 詳細
| RRC ID | 30195 |
|---|---|
| 著者 | Mizuno K, Shiba K, Okai M, Takahashi Y, Shitaka Y, Oiwa K, Tanokura M, Inaba K. |
| タイトル | Calaxin drives sperm chemotaxis by Ca²⁺-mediated direct modulation of a dynein motor. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Sperm chemotaxis occurs widely in animals and plants and plays an important role in the success of fertilization. Several studies have recently demonstrated that Ca(2+) influx through specific Ca(2+) channels is a prerequisite for sperm chemotactic movement. However, the regulator that modulates flagellar movement in response to Ca(2+) is unknown. Here we show that a neuronal calcium sensor, calaxin, directly acts on outer-arm dynein and regulates specific flagellar movement during sperm chemotaxis. Calaxin inhibition resulted in significant loss of sperm chemotactic movement, despite normal increases in intracellular calcium concentration. Using a demembranated sperm model, we demonstrate that calaxin is essential for generation and propagation of Ca(2+)-induced asymmetric flagellar bending. An in vitro motility assay revealed that calaxin directly suppressed the velocity of microtubule sliding by outer-arm dynein at high Ca(2+) concentrations. This study describes the missing link between chemoattractant-mediated Ca(2+) signaling and motor-driven microtubule sliding during sperm chemotaxis. |
| 巻・号 | 109(50) |
| ページ | 20497-502 |
| 公開日 | 2012-12-11 |
| DOI | 10.1073/pnas.1217018109 |
| PII | 1217018109 |
| PMID | 23169663 |
| PMC | PMC3528578 |
| MeSH | Animals Calcium Signaling / physiology Carbamates / pharmacology Chemotaxis / drug effects Chemotaxis / physiology Ciona intestinalis / cytology Ciona intestinalis / physiology Dyneins / physiology* Intracellular Calcium-Sensing Proteins / physiology* Male Microtubules / physiology Models, Biological Molecular Motor Proteins / physiology Piperidines / pharmacology Sperm Motility / physiology Sperm Tail / physiology Spermatozoa / drug effects Spermatozoa / physiology* |
| IF | 9.412 |
| 引用数 | 41 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |