論文 - 詳細
| RRC ID | 30212 |
|---|---|
| 著者 | Dowling MR, Kan A, Heinzel S, Zhou JH, Marchingo JM, Wellard CJ, Markham JF, Hodgkin PD. |
| タイトル | Stretched cell cycle model for proliferating lymphocytes. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Stochastic variation in cell cycle time is a consistent feature of otherwise similar cells within a growing population. Classic studies concluded that the bulk of the variation occurs in the G1 phase, and many mathematical models assume a constant time for traversing the S/G2/M phases. By direct observation of transgenic fluorescent fusion proteins that report the onset of S phase, we establish that dividing B and T lymphocytes spend a near-fixed proportion of total division time in S/G2/M phases, and this proportion is correlated between sibling cells. This result is inconsistent with models that assume independent times for consecutive phases. Instead, we propose a stretching model for dividing lymphocytes where all parts of the cell cycle are proportional to total division time. Data fitting based on a stretched cell cycle model can significantly improve estimates of cell cycle parameters drawn from DNA labeling data used to monitor immune cell dynamics. |
| 巻・号 | 111(17) |
| ページ | 6377-82 |
| 公開日 | 2014-4-29 |
| DOI | 10.1073/pnas.1322420111 |
| PII | 1322420111 |
| PMID | 24733943 |
| PMC | PMC4036001 |
| MeSH | Animals B-Lymphocytes / cytology* Bromodeoxyuridine / metabolism Cell Cycle* Cell Division Cell Proliferation DNA / metabolism Fluorescence Genes, Reporter Mice Mice, Inbred C57BL Models, Immunological Probability T-Lymphocytes / cytology* Time Factors |
| IF | 9.412 |
| 引用数 | 43 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC02705 RBRC02709 |