| RRC ID |
30212
|
| Author |
Dowling MR, Kan A, Heinzel S, Zhou JH, Marchingo JM, Wellard CJ, Markham JF, Hodgkin PD.
|
| Title |
Stretched cell cycle model for proliferating lymphocytes.
|
| Journal |
Proc Natl Acad Sci U S A
|
| Abstract |
Stochastic variation in cell cycle time is a consistent feature of otherwise similar cells within a growing population. Classic studies concluded that the bulk of the variation occurs in the G1 phase, and many mathematical models assume a constant time for traversing the S/G2/M phases. By direct observation of transgenic fluorescent fusion proteins that report the onset of S phase, we establish that dividing B and T lymphocytes spend a near-fixed proportion of total division time in S/G2/M phases, and this proportion is correlated between sibling cells. This result is inconsistent with models that assume independent times for consecutive phases. Instead, we propose a stretching model for dividing lymphocytes where all parts of the cell cycle are proportional to total division time. Data fitting based on a stretched cell cycle model can significantly improve estimates of cell cycle parameters drawn from DNA labeling data used to monitor immune cell dynamics.
|
| Volume |
111(17)
|
| Pages |
6377-82
|
| Published |
2014-4-29
|
| DOI |
10.1073/pnas.1322420111
|
| PII |
1322420111
|
| PMID |
24733943
|
| PMC |
PMC4036001
|
| MeSH |
Animals
B-Lymphocytes / cytology*
Bromodeoxyuridine / metabolism
Cell Cycle*
Cell Division
Cell Proliferation
DNA / metabolism
Fluorescence
Genes, Reporter
Mice
Mice, Inbred C57BL
Models, Immunological
Probability
T-Lymphocytes / cytology*
Time Factors
|
| IF |
9.412
|
| Times Cited |
43
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| Mice |
RBRC02705
RBRC02709 |
