論文 - 詳細
| RRC ID | 30216 |
|---|---|
| 著者 | Olson LJ, Orsi R, Alculumbre SG, Peterson FC, Stigliano ID, Parodi AJ, D'Alessio C, Dahms NM. |
| タイトル | Structure of the lectin mannose 6-phosphate receptor homology (MRH) domain of glucosidase II, an enzyme that regulates glycoprotein folding quality control in the endoplasmic reticulum. |
| ジャーナル | J Biol Chem |
| Abstract |
Here we report for the first time the three-dimensional structure of a mannose 6-phosphate receptor homology (MRH) domain present in a protein with enzymatic activity, glucosidase II (GII). GII is involved in glycoprotein folding in the endoplasmic reticulum. GII removes the two innermost glucose residues from the Glc3Man9GlcNAc2 transferred to nascent proteins and the glucose added by UDP-Glc:glycoprotein glucosyltransferase. GII is composed of a catalytic GIIα subunit and a regulatory GIIβ subunit. GIIβ participates in the endoplasmic reticulum localization of GIIα and mediates in vivo enhancement of N-glycan trimming by GII through its C-terminal MRH domain. We determined the structure of a functional GIIβ MRH domain by NMR spectroscopy. It adopts a β-barrel fold similar to that of other MRH domains, but its binding pocket is the most shallow known to date as it accommodates a single mannose residue. In addition, we identified a conserved residue outside the binding pocket (Trp-409) present in GIIβ but not in other MRHs that influences GII glucose trimming activity. |
| 巻・号 | 288(23) |
| ページ | 16460-16475 |
| 公開日 | 2013-6-7 |
| DOI | 10.1074/jbc.M113.450239 |
| PII | S0021-9258(20)53655-7 |
| PMID | 23609449 |
| PMC | PMC3675582 |
| MeSH | Crystallography, X-Ray Endoplasmic Reticulum* / chemistry Endoplasmic Reticulum* / genetics Endoplasmic Reticulum* / metabolism Glycoproteins* / chemistry Glycoproteins* / genetics Glycoproteins* / metabolism Mannose / chemistry Mannose / genetics Mannose / metabolism Protein Folding* Protein Structure, Secondary Protein Structure, Tertiary Schizosaccharomyces / enzymology* Schizosaccharomyces / genetics Schizosaccharomyces pombe Proteins* / chemistry Schizosaccharomyces pombe Proteins* / genetics Schizosaccharomyces pombe Proteins* / metabolism alpha-Glucosidases* / chemistry alpha-Glucosidases* / genetics alpha-Glucosidases* / metabolism |
| IF | 4.238 |
| 引用数 | 21 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | S. pombe entry SpEnt26D11 (SPW010483). |