論文 - 詳細
| RRC ID | 3022 |
|---|---|
| 著者 | Rivera WL, Santos SR, Kanbara H. |
| タイトル | Prevalence and genetic diversity of Entamoeba histolytica in an institution for the mentally retarded in the Philippines. |
| ジャーナル | Parasitol Res |
| Abstract |
A total of 113 mentally retarded patients residing in a mental institution in Metropolitan Manila, Philippines, were screened for the presence of Entamoeba histolytica based on microscopy and polymerase chain reaction (PCR). Anti-E. histolytica antibodies were also screened in 97 serum samples collected using immunofluorescence antibody (IFA) test. Parasitological examination showed E. histolytica/Entamoeba dispar in 43 cases (38.05%), while PCR detected 74 cases (65.48%) positive for E. histolytica and 6 cases (5.30%) positive for E. dispar. Interestingly, these 6 samples were coinfected with E. histolytica. IFA test revealed that 80.41% (78/97) of the respondents possessed significant antibody titers for intestinal infection of E. histolytica. Of this number, there were 5 patients negative in IFA test but positive in PCR. The genetic diversity of E. histolytica isolates was also investigated by analyzing polymorphism in the serine-rich gene by nested PCR on DNA directly extracted from stool specimens. A combination of the nested PCR results and the AluI digestion of the PCR products examined yielded six distinct DNA banding patterns among the 74 stool isolates. An apparent clustering of E. histolytica strains was observed in patients living in different residential cottages of the institution. These results indicate the high prevalence of E. histolytica in an institution for the mentally retarded in the Philippines. |
| 巻・号 | 98(2) |
| ページ | 106-10 |
| 公開日 | 2006-1-1 |
| DOI | 10.1007/s00436-005-0024-8 |
| PMID | 16284779 |
| MeSH | Animals Antibodies, Protozoan / blood DNA, Protozoan / analysis Entamoeba / classification Entamoeba / genetics Entamoeba / immunology Entamoeba / isolation & purification Entamoeba histolytica / classification* Entamoeba histolytica / genetics Entamoeba histolytica / immunology Entamoeba histolytica / isolation & purification Entamoebiasis / epidemiology* Entamoebiasis / parasitology Feces / parasitology Genetic Variation* Health Facilities* Humans Persons with Mental Disabilities* Philippines / epidemiology Polymerase Chain Reaction Prevalence |
| IF | 1.641 |
| 引用数 | 32 |
| WOS 分野 | PARASITOLOGY |
| リソース情報 | |
| 病原微生物 | E. histolytica? |