| RRC ID |
3028
|
| 著者 |
Sakuma K, Kitahara M, Kibe R, Sakamoto M, Benno Y.
|
| タイトル |
Clostridium glycyrrhizinilyticum sp. nov., a glycyrrhizin-hydrolysing bacterium isolated from human faeces.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
Screening of faecal bacteria for glycyrrhetic acid (GA) production by hydrolysing of glycyrrhizin (GL) resulted in the isolation of two strains, designated ZM35T and ZM38. Strains ZM35T and ZM38 were Gram-positive, obligate anaerobic, non-spore-forming and rod-shaped bacteria. Analysis of the 16S rRNA gene sequences indicated that strains ZM35T and ZM38 belonged to cluster XIVa of the genus Clostridium. The 16S rRNA gene sequences of strains ZM35T and ZM38 were identical. Strain ZM35T exhibited approximately 94% to 95% identity with the validly described species, Clostridium oroticum(94.5%), Eubacterium contortum(93.8%), Ruminococcus gnavus(94.5%) and R. torques(95.1%). In an experiment of DNA-DNA hybridization, it was confirmed that strains ZM35T and ZM38 were the same species. The guanine-plus-cytosine (G+C) content of strain ZM35T is 45.7 mol%. Based on the phylogenetic and phenotypic findings, we propose that strains ZM35T and ZM38 be assigned to a novel species named Clostridium glycyrrhizinilyticum. The type strain is ZM35T (=JCM 13368T=DSM 17593T).
|
| 巻・号 |
50(7)
|
| ページ |
481-5
|
| 公開日 |
2006-1-1
|
| DOI |
10.1111/j.1348-0421.2006.tb03818.x
|
| PII |
JST.JSTAGE/mandi/50.481
|
| PMID |
16858139
|
| MeSH |
Clostridium / classification*
Clostridium / genetics
Clostridium / isolation & purification
Clostridium / metabolism
Feces / microbiology
Glycyrrhetinic Acid / metabolism
Glycyrrhizic Acid / metabolism*
Humans
Hydrolysis
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
|
| IF |
1.566
|
| 引用数 |
12
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
JCM 13368? |
