RRC ID 30357
著者 Nishida K, Takahara M, Miyagishima SY, Kuroiwa H, Matsuzaki M, Kuroiwa T.
タイトル Dynamic recruitment of dynamin for final mitochondrial severance in a primitive red alga.
ジャーナル Proc Natl Acad Sci U S A
Abstract Dynamins are a eukaryote-specific family of GTPases. Some family members are involved in diverse and varied cellular activities. Here, we report that the primitive red alga Cyanidioschyzon merolae retains only one dynamin homolog, CmDnm1, belonging to the mitochondrial division subfamily. Previously, the bacterial cell division protein, FtsZ, was shown to localize at the mitochondrial division site in the alga. We showed that FtsZ and dynamin coexist as mitochondrial division-associated proteins that act during different phases of division. CmDnm1 was recruited from 10-20 cytoplasmic patches (dynamin patches) to the midpoint of the constricted mitochondrion-dividing ring (MD ring), which was observed as an electron-dense structure on the cytoplasmic side. CmDnm1 is probably not required for early constriction; it forms a ring or spiral when the outer mitochondrial membrane is finally severed, whereas the FtsZ and MD rings are formed before constriction. It is thought that the FtsZ, MD, and dynamin rings are involved in scaffolding, constriction, and final separation, respectively. In eukaryotes, mitochondrial severance is probably the most conserved role for the dynamin family.
巻・号 100(4)
ページ 2146-51
公開日 2003-2-18
DOI 10.1073/pnas.0436886100
PII 0436886100
PMID 12566569
PMC PMC149973
MeSH Blotting, Western Dynamins / metabolism* Microscopy, Electron Microscopy, Fluorescence Mitochondria / metabolism* Molecular Sequence Data Rhodophyta / metabolism* Rhodophyta / ultrastructure
IF 9.412
引用数 100
WOS 分野 CELL BIOLOGY
リソース情報
藻類 NIES-3377