RRC ID 30373
Author Fujiwara T, Misumi O, Tashiro K, Yoshida Y, Nishida K, Yagisawa F, Imamura S, Yoshida M, Mori T, Tanaka K, Kuroiwa H, Kuroiwa T.
Title Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga Cyanidioschyzon merolae.
Journal DNA Res
Abstract Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.
Volume 16(1)
Pages 59-72
Published 2009-2-1
DOI 10.1093/dnares/dsn032
PII dsn032
PMID 19147531
PMC PMC2646357
MeSH Algal Proteins / genetics Algal Proteins / metabolism Cell Cycle / genetics* Cell Division / genetics* Cell Nucleus / metabolism* Gene Expression* Mitochondria / metabolism Organelles / metabolism Plastids / metabolism Rhodophyta / cytology Rhodophyta / genetics* Rhodophyta / metabolism
IF 4.009
Times Cited 47
Algae NIES-3377