RRC ID 30376
Author Yoshida Y, Kuroiwa H, Misumi O, Nishida K, Yagisawa F, Fujiwara T, Nanamiya H, Kawamura F, Kuroiwa T.
Title Isolated chloroplast division machinery can actively constrict after stretching.
Journal Science
Abstract Chloroplast division involves plastid-dividing, dynamin, and FtsZ (PDF) rings. We isolated intact supertwisted (or spiral) and circular PDF machineries from chloroplasts of the red alga Cyanidioschyzon merolae. After individual intact PDF machineries were stretched to four times their original lengths with optical tweezers, they spontaneously returned to their original sizes. Dynamin-released PDF machineries did not retain the spiral structure and could not be stretched. Thus, dynamin may generate the motive force for contraction by filament sliding in dividing chloroplasts, in addition to pinching-off the membranes.
Volume 313(5792)
Pages 1435-8
Published 2006-9-8
DOI 10.1126/science.1129689
PII 313/5792/1435
PMID 16960006
MeSH Actin Cytoskeleton / physiology* Actin Cytoskeleton / ultrastructure Algal Proteins / physiology* Chloroplasts / physiology* Chloroplasts / ultrastructure Dynamins / physiology* GTP Phosphohydrolases / physiology Intracellular Membranes / physiology Intracellular Membranes / ultrastructure Microscopy, Immunoelectron Rhodophyta / physiology* Rhodophyta / ultrastructure
IF 41.846
Times Cited 68
WOS Category CELL BIOLOGY
Resource
Algae NIES-3377