論文 - 詳細
| RRC ID | 30385 |
|---|---|
| 著者 | Ohnuma M, Misumi O, Fujiwara T, Watanabe S, Tanaka K, Kuroiwa T. |
| タイトル | Transient gene suppression in a red alga, Cyanidioschyzon merolae 10D. |
| ジャーナル | Protoplasma |
| Abstract |
Antisense suppression is a powerful tool to analyze gene function. In this study, we show that antisense RNA suppressed the expression of a target gene in the unicellular red alga, Cyanidioschyzon merolae. In this study, the antisense strand of the catalase gene was cloned and inserted into an expression vector upstream of the GFP gene. This plasmid was introduced into C. merolae cells using a polyethylene glycol-mediated transformation protocol. Using the expression of GFP as a marker of transformed cells, the expression of catalase was examined by immunocytochemistry. Decreased expression of catalase was observed in cells that were transformed with the antisense strand of the catalase gene. These results indicate the utility of this antisense suppression system. |
| 巻・号 | 236(1-4) |
| ページ | 107-12 |
| 公開日 | 2009-7-1 |
| DOI | 10.1007/s00709-009-0056-5 |
| PMID | 19533298 |
| MeSH | Catalase / genetics Catalase / metabolism Gene Expression Regulation, Enzymologic* Immunohistochemistry RNA, Antisense / genetics RNA, Antisense / physiology Rhodophyta / enzymology Rhodophyta / genetics* |
| IF | 2.751 |
| 引用数 | 33 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-3377 |