RRC ID 30385
著者 Ohnuma M, Misumi O, Fujiwara T, Watanabe S, Tanaka K, Kuroiwa T.
タイトル Transient gene suppression in a red alga, Cyanidioschyzon merolae 10D.
ジャーナル Protoplasma
Abstract Antisense suppression is a powerful tool to analyze gene function. In this study, we show that antisense RNA suppressed the expression of a target gene in the unicellular red alga, Cyanidioschyzon merolae. In this study, the antisense strand of the catalase gene was cloned and inserted into an expression vector upstream of the GFP gene. This plasmid was introduced into C. merolae cells using a polyethylene glycol-mediated transformation protocol. Using the expression of GFP as a marker of transformed cells, the expression of catalase was examined by immunocytochemistry. Decreased expression of catalase was observed in cells that were transformed with the antisense strand of the catalase gene. These results indicate the utility of this antisense suppression system.
巻・号 236(1-4)
ページ 107-12
公開日 2009-7-1
DOI 10.1007/s00709-009-0056-5
PMID 19533298
MeSH Catalase / genetics Catalase / metabolism Gene Expression Regulation, Enzymologic* Immunohistochemistry RNA, Antisense / genetics RNA, Antisense / physiology Rhodophyta / enzymology Rhodophyta / genetics*
IF 2.751
引用数 33
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
藻類 NIES-3377