論文 - 詳細
| RRC ID | 30428 |
|---|---|
| 著者 | Verkhusha VV, Tsukita S, Oda H. |
| タイトル | Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein. |
| ジャーナル | FEBS Lett |
| Abstract |
Directional migration of border cells in the Drosophila egg chambers is a developmentally regulated event that requires dynamic cellular functions. In this study, the electron microscopic observation of migrating border cells revealed loose actin bundles in forepart lamellipodia and numerous microvilli extending from nurse cells and providing multiple adhesive contacts with border cells. To analyze the dynamics of actin in migrating border cells in vivo, we constructed a green fluorescent protein-actin fusion protein and induced its expression in Drosophila using the GAL4/UAS system. The green fluorescent protein-actin was incorporated into the actin bundles and it enabled visualization of the rapid cytoskeletal changes in border cell lamellipodia. During the growth of the lamellipodia, the actin bundles that increased in number and size radiated from the bundle-organizing center. Quantification of the fluorescence intensity showed that an accumulation of bundle-associated and spotted green fluorescent protein-actin signals took place during their centripetal movement. Our results favored a treadmilling model for actin behavior in border cell lamellipodia. |
| 巻・号 | 445(2-3) |
| ページ | 395-401 |
| 公開日 | 1999-2-26 |
| DOI | 10.1016/s0014-5793(99)00124-6 |
| PII | S0014-5793(99)00124-6 |
| PMID | 10094496 |
| MeSH | Actins / genetics Actins / metabolism* Actins / ultrastructure Animals Cell Movement* Drosophila Female Green Fluorescent Proteins Luminescent Proteins / genetics Luminescent Proteins / metabolism Luminescent Proteins / ultrastructure Ovary / cytology* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Recombinant Fusion Proteins / ultrastructure |
| IF | 3.057 |
| 引用数 | 93 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | DGRC#109008 |