Reference - Detail
| RRC ID | 30447 |
|---|---|
| Author | Okada M, Akimaru H, Hou DX, Takahashi T, Ishii S. |
| Title | Myb controls G(2)/M progression by inducing cyclin B expression in the Drosophila eye imaginal disc. |
| Journal | EMBO J |
| Abstract |
The c-myb proto-oncogene product (c-Myb) is a transcriptional activator. Vertebrate c-Myb is a key regulator of the G(1)/S transition in cell cycle, while Drosophila Myb (dMyb) is important for the G(2)/M transition. Here we report that dMyb induces expression of cyclin B, a critical regulator of the G(2)/M transition, in Drosophila eye imaginal disc. In the wild-type eye disc, dmyb mRNA was expressed in the stripes both anterior and posterior to the morphogenetic furrow. Ectopic expression of C-terminal-truncated dMyb in the eye disc caused ectopic expression of cyclin B and the rough eye phenotype. This rough eye phenotype correlated with prolonged M phase, caused by overexpression of cyclin B. Cyclin B expression was lost in dmyb-deficient clones. In Schneider cells, the activity of the cyclin B promoter was dramatically reduced by loss of dMyb using the RNA interference method. Mutations of the multiple AACNG sequences in the cyclin B promoter also abolished the promoter activity. These results indicate that dMyb regulates the G(2)/M transition by inducing cyclin B expression via binding to its promoter. |
| Volume | 21(4) |
| Pages | 675-84 |
| Published | 2002-2-15 |
| DOI | 10.1093/emboj/21.4.675 |
| PMID | 11847115 |
| PMC | PMC125351 |
| MeSH | Animals Animals, Genetically Modified Base Sequence Cyclin B / genetics* DNA Primers Drosophila Drosophila Proteins Eye / metabolism* Eye / ultrastructure G2 Phase / physiology* Gene Expression Regulation / physiology* Microscopy, Electron, Scanning Mitosis / physiology* Proto-Oncogene Proteins c-myb / physiology* Reverse Transcriptase Polymerase Chain Reaction Transcriptional Activation / physiology |
| IF | 9.889 |
| Times Cited | 59 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#109167 |