| RRC ID |
30568
|
| 著者 |
Yamamoto K, Suzuki M, Higashiura A, Aritake K, Urade Y, Uodome N, Hossain T, Nakagawa A.
|
| タイトル |
New insights into the catalytic mechanism of Bombyx mori prostaglandin E synthase gained from structure-function analysis.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Prostaglandin E synthase (PGES) catalyzes the isomerization of PGH2 to PGE2. We previously reported the identification and structural characterization of Bombyx mori PGES (bmPGES), which belongs to Sigma-class glutathione transferase. Here, we extend these studies by determining the structure of bmPGES in complex with glutathione sulfonic acid (GTS) at a resolution of 1.37 Å using X-ray crystallography. GTS localized to the glutathione-binding site. We found that electron-sharing network of bmPGES includes Asn95, Asp96, and Arg98. Site-directed mutagenesis of these residues to create mutant forms of bmPGES mutants indicate that they contribute to catalytic activity. These results are, to our knowledge, the first to reveal the presence of an electron-sharing network in bmPGES.
|
| 巻・号 |
440(4)
|
| ページ |
762-7
|
| 公開日 |
2013-11-1
|
| DOI |
10.1016/j.bbrc.2013.10.001
|
| PII |
S0006-291X(13)01655-0
|
| PMID |
24120949
|
| MeSH |
Animals
Arginine / chemistry
Arginine / genetics
Binding Sites
Bombyx / enzymology*
Catalysis
Crystallography, X-Ray
Electrons
Glutathione / analogs & derivatives*
Glutathione / chemistry
Intramolecular Oxidoreductases / chemistry*
Intramolecular Oxidoreductases / genetics
Mutagenesis, Site-Directed
Prostaglandin-E Synthases
Protein Conformation
|
| IF |
2.985
|
| 引用数 |
6
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
|
