論文 - 詳細
| RRC ID | 30577 |
|---|---|
| 著者 | Mon H, Li Z, Kobayashi I, Tomita S, Lee J, Sezutsu H, Tamura T, Kusakabe T. |
| タイトル | Soaking RNAi in Bombyx mori BmN4-SID1 cells arrests cell cycle progression. |
| ジャーナル | J Insect Sci |
| Abstract |
RNA interference (RNAi) is an evolutionarily conserved mechanism for sequence-specific gene silencing. Previously, the BmN4-SID1 cell expressing Caenorhabditis ele gans SID-1 was established, in which soaking RNAi could induce effective gene silencing. To establish its utility, 6 cell cycle progression related cDNAs, CDK1, MYC, MYB, RNRS, CDT1, and GEMININ, were isolated from the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), and their expressions were further silenced by soaking RNAi in the BmN4-SID1 cells. The cell cycle progression analysis using flow cytometer demonstrated that the small amount of double stranded RNA was enough to arrest cell cycle progression at the specific cell phases. These data suggest that RNAi in the BmN4-SID1 cells can be used as a powerful tool for loss-of-function analysis of B. mori genes. |
| 巻・号 | 13 |
| ページ | 155 |
| 公開日 | 2013-1-1 |
| DOI | 10.1673/031.013.15501 |
| PMID | 24773378 |
| PMC | PMC4015410 |
| MeSH | Animals Animals, Genetically Modified / genetics Animals, Genetically Modified / physiology Bombyx / cytology* Bombyx / genetics Bombyx / physiology* Caenorhabditis elegans Cell Cycle* Cell Line DNA, Complementary / genetics DNA, Complementary / metabolism Flow Cytometry Gene Knockdown Techniques Insect Proteins / genetics* Insect Proteins / metabolism Molecular Sequence Data RNA Interference* RNA, Double-Stranded / genetics RNA, Double-Stranded / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, RNA |
| IF | 1.325 |
| 引用数 | 10 |
| WOS 分野 | ENTOMOLOGY |
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