RRC ID 30596
Author Nakamoto M, Fukasawa M, Tanaka S, Shimamori K, Suzuki A, Matsuda M, Kobayashi T, Nagahama Y, Shibata N.
Title Expression of 3β-hydroxysteroid dehydrogenase (hsd3b), star and ad4bp/sf-1 during gonadal development in medaka (Oryzias latipes).
Journal Gen. Comp. Endocrinol.
Abstract In most vertebrates, sex steroids play a critical role in gonadal development, maturation of germ cells, and development of secondary sexual characteristics. Sex steroids are synthesized in steroid-producing cells (SPCs) in the testis known as Leydig cells, as well as in thecal and granulosa cells in the ovary. In SPCs, cholesterol is sequentially catalyzed by a set of steroidogenic factors and enzymes in order to produce sex steroids. Therefore, integrated expression of the genes involved in steroidogenesis is critical for the proper production of sex steroids. In the present study, regulatory mechanisms of steroidogenic factors and enzymes were examined. We focused on hsd3b, star and ad4bp/sf-1 as well as the description of temporal and spatial expression of these genes during gonadal development in medaka (Oryzias latipes). During testicular development, hsd3b, star and ad4bp/sf-1 were co-expressed in the interstitial somatic cells subsequent to the formation of the seminiferous tubule precursor, suggesting that ad4bp/sf-1 regulated the transcription of both hsd3b and star. During ovarian development, the expression pattern of hsd3b coincided with that of cyp11a1, but not with that of aromatase. Although ad4bp/sf-1 was mainly expressed in presumptive follicular cells, it was also detected in hsd3b positive interstitial cells in the developing ovary. Contrary to our expectations, the onset of star expression occurred during a later stage of ovarian development than the expression of other steroidogenic enzymes. Thus, the regulation mechanism of star transcription appears to differ from that of the other steroidogenic enzymes in the developing ovary, but not in the developing testis.
Volume 176(2)
Pages 222-30
Published 2012-4-1
DOI 10.1016/j.ygcen.2012.01.019
PII S0016-6480(12)00046-9
PMID 22330050
MeSH 17-Hydroxysteroid Dehydrogenases / chemistry 17-Hydroxysteroid Dehydrogenases / genetics* 17-Hydroxysteroid Dehydrogenases / metabolism Amino Acid Sequence Animals Gene Expression Regulation, Developmental Gonads / metabolism* In Situ Hybridization, Fluorescence Membrane Proteins / chemistry Membrane Proteins / genetics* Membrane Proteins / metabolism Molecular Sequence Data Oryzias / metabolism* Sequence Homology, Amino Acid Steroidogenic Factor 1 / chemistry Steroidogenic Factor 1 / genetics* Steroidogenic Factor 1 / metabolism
IF 2.564
Times Cited 11
WOS Category ENDOCRINOLOGY & METABOLISM
Resource
Medaka d-rR/TOKYO (MT837) HdrR-II1 (IB178)