論文 - 詳細
| RRC ID | 30605 |
|---|---|
| 著者 | Kato Y, Kusakabe R, Inoue K, Tochinai S. |
| タイトル | MiR-124 is involved in post-transcriptional regulation of Polypyrimidine tract binding protein 1 (PTBP1) during neural development in the medaka, Oryzias latipes. |
| ジャーナル | Zoolog Sci |
| Abstract |
MicroRNAs (miRNAs) comprise a group of small noncoding RNA molecules thought to have contributed to the evolution of vertebrate brain homogeneity and diversity. The miRNA miR-124 is well conserved between invertebrates and vertebrates and is expressed abundantly in the central nervous system (CNS). We identified miR-124 in the medaka, Oryzias latipes, and investigated its role in neural development. The five candidate genes for medaka precursor miR-124 are unlinked on four different chromosomes and differ in nucleotide length. Their sequences suggest that they can generate functional miRNAs through conventional miRNA biogenesis by folding into stem-loop structures. Whole-mount in situ hybridization and northern blotting revealed that mature miR-124 is specifically expressed in the CNS and the eyes starting at two days post-fertilization. We also examined the sequences and expression of medaka Polypyrimidine tract binding protein 1 (Ptbp1), a possible direct target of miR-124. The 3'UTR of medaka Ptbp1 contains predicted binding motifs (target sites) for miR-124. A GFP reporter assay for the target sites or the entire 3'UTR showed that exogenous miR-124 silences PTBP1 expression in vivo. Our study suggests that medaka miR-124 is involved in post-transcriptional regulation of target genes in neural development and that medaka miR-124 homologs may have spatiotemporal roles different from those in other vertebrates. |
| 巻・号 | 30(11) |
| ページ | 891-900 |
| 公開日 | 2013-11-1 |
| DOI | 10.2108/zsj.30.891 |
| PMID | 24199853 |
| MeSH | Amino Acid Sequence Animals Base Sequence Embryo, Nonmammalian / metabolism Embryonic Development / physiology Gene Expression Regulation, Developmental / physiology* MicroRNAs / genetics MicroRNAs / metabolism* Molecular Sequence Data Nucleic Acid Conformation Oryzias / embryology* Polypyrimidine Tract-Binding Protein / genetics Polypyrimidine Tract-Binding Protein / metabolism* RNA Processing, Post-Transcriptional / physiology* |
| IF | 0.843 |
| 引用数 | 1 |
| WOS 分野 | ZOOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| メダカ | hatching enzyme cDNA |