| RRC ID |
30627
|
| 著者 |
Ashe A, Sapetschnig A, Weick EM, Mitchell J, Bagijn MP, Cording AC, Doebley AL, Goldstein LD, Lehrbach NJ, Le Pen J, Pintacuda G, Sakaguchi A, Sarkies P, Ahmed S, Miska EA.
|
| タイトル |
piRNAs can trigger a multigenerational epigenetic memory in the germline of C. elegans.
|
| ジャーナル |
Cell
|
| Abstract |
Transgenerational effects have wide-ranging implications for human health, biological adaptation, and evolution; however, their mechanisms and biology remain poorly understood. Here, we demonstrate that a germline nuclear small RNA/chromatin pathway can maintain stable inheritance for many generations when triggered by a piRNA-dependent foreign RNA response in C. elegans. Using forward genetic screens and candidate approaches, we find that a core set of nuclear RNAi and chromatin factors is required for multigenerational inheritance of environmental RNAi and piRNA silencing. These include a germline-specific nuclear Argonaute HRDE1/WAGO-9, a HP1 ortholog HPL-2, and two putative histone methyltransferases, SET-25 and SET-32. piRNAs can trigger highly stable long-term silencing lasting at least 20 generations. Once established, this long-term memory becomes independent of the piRNA trigger but remains dependent on the nuclear RNAi/chromatin pathway. Our data present a multigenerational epigenetic inheritance mechanism induced by piRNAs.
|
| 巻・号 |
150(1)
|
| ページ |
88-99
|
| 公開日 |
2012-7-6
|
| DOI |
10.1016/j.cell.2012.06.018
|
| PII |
S0092-8674(12)00769-6
|
| PMID |
22738725
|
| PMC |
PMC3464430
|
| MeSH |
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism
Cell Nucleus / genetics
Cell Nucleus / metabolism
Epigenomics*
Female
Germ Cells / metabolism
Male
RNA Interference*
RNA, Helminth / metabolism*
RNA, Small Interfering / metabolism*
Transgenes
|
| IF |
38.637
|
| 引用数 |
373
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm1200 |
