RRC ID 30647
著者 Ji N, Middelkoop TC, Mentink RA, Betist MC, Tonegawa S, Mooijman D, Korswagen HC, van Oudenaarden A.
タイトル Feedback control of gene expression variability in the Caenorhabditis elegans Wnt pathway.
ジャーナル Cell
Abstract Variability in gene expression contributes to phenotypic heterogeneity even in isogenic populations. Here, we used the stereotyped, Wnt signaling-dependent development of the Caenorhabditis elegans Q neuroblast to probe endogenous mechanisms that control gene expression variability. We found that the key Hox gene that orients Q neuroblast migration exhibits increased gene expression variability in mutants in which Wnt pathway activity has been perturbed. Distinct features of the gene expression distributions prompted us on a systematic search for regulatory interactions, revealing a network of interlocked positive and negative feedback loops. Interestingly, positive feedback appeared to cooperate with negative feedback to reduce variability while keeping the Hox gene expression at elevated levels. A minimal model correctly predicts the increased gene expression variability across mutants. Our results highlight the influence of gene network architecture on expression variability and implicate feedback regulation as an effective mechanism to ensure developmental robustness.
巻・号 155(4)
ページ 869-80
公開日 2013-11-7
DOI 10.1016/j.cell.2013.09.060
PII S0092-8674(13)01279-8
PMID 24209624
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Cell Movement Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Feedback, Physiological Frizzled Receptors / genetics Frizzled Receptors / metabolism Gene Regulatory Networks Genetic Variation* Glycoproteins / genetics Homeodomain Proteins / genetics Transcription Factors / genetics Wnt Proteins Wnt Signaling Pathway*
IF 38.637
引用数 50
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm1320