論文 - 詳細
| RRC ID | 30664 |
|---|---|
| 著者 | Mise-Omata S, Alles N, Fukazawa T, Aoki K, Ohya K, Jimi E, Obata Y, Doi T. |
| タイトル | NF-κB RELA-deficient bone marrow macrophages fail to support bone formation and to maintain the hematopoietic niche after lethal irradiation and stem cell transplantation. |
| ジャーナル | Int Immunol |
| Abstract |
Bone remodeling and hematopoiesis are interrelated and bone marrow (BM) macrophages are considered to be important for both bone remodeling and maintenance of the hematopoietic niche. We found that NF-κB Rela-deficient chimeric mice, generated by transplanting Rela (-/-) fetal liver cells into lethally irradiated hosts, developed severe osteopenia, reduced lymphopoiesis and enhanced mobilization of hematopoietic stem and progenitor cells when BM cells were completely substituted by Rela-deficient cells. Rela (-/-) hematopoietic stem cells from fetal liver had normal hematopoietic ability, but those harvested from the BM of osteopenic Rela (-/-) chimeric mice had reduced repopulation ability, indicating impairment of the microenvironment for the hematopoietic niche. Osteopenia in Rela (-/-) chimeric mice was due to reduced bone formation, even though osteoblasts differentiated from host cells. This finding indicates impaired functional coupling between osteoblasts and hematopoietic stem cell-derived cells. Rela-deficient BM macrophages exhibited an aberrant inflammatory phenotype, and transplantation with wild-type F4/80(+) BM macrophages recovered bone formation and ameliorated lymphopoiesis in Rela (-/-) chimeric mice. Therefore, RELA in F4/80(+) macrophages is important both for bone homeostasis and for maintaining the hematopoietic niche after lethal irradiation and hematopoietic stem cell transplantation. |
| 巻・号 | 26(11) |
| ページ | 607-18 |
| 公開日 | 2014-11-1 |
| DOI | 10.1093/intimm/dxu062 |
| PII | dxu062 |
| PMID | 24908679 |
| MeSH | Animals Bone Diseases, Metabolic / genetics Bone Diseases, Metabolic / metabolism Bone Diseases, Metabolic / pathology Bone Marrow / metabolism Bone Marrow / pathology Hematopoiesis / genetics* Hematopoietic Stem Cell Transplantation Lymphopoiesis / genetics Macrophages / metabolism* Male Mice Mice, Knockout Osteoclasts / metabolism Osteogenesis / genetics* Stem Cell Niche / genetics* Transcription Factor RelA / deficiency* Transcription Factor RelA / genetics Transplantation Chimera Whole-Body Irradiation |
| IF | 3.519 |
| 引用数 | 8 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00144 |