RRC ID 30688
Author Basset A, Tzou P, Lemaitre B, Boccard F.
Title A single gene that promotes interaction of a phytopathogenic bacterium with its insect vector, Drosophila melanogaster.
Journal EMBO Rep
Abstract Insects are major vectors of plant and animal disease, and bacterial phytopathogens are often disseminated by flies. We have previously reported that some isolates of the phytopathogenic bacterial species Erwinia carotovora infect Drosophila and activate an immune response. Using a genetic screen, we have now identified two genes that are required by E. carotovora to infect Drosophila. One of these genes has a regulatory role whereas the other, evf, confers an infectious phenotype: its transfer to non-infectious Erwinia strains or to several enterobacteria improves survival in the gut and triggers the immune response. Overexpression of Erwinia virulence factor (evf) allowed bacteria to colonize the apical side of the gut epithelium and in some cases to spread to the body cavity. Our results demonstrate a specific interaction between plant pathogens and flies that promote their dissemination.
Volume 4(2)
Pages 205-9
Published 2003-2-1
DOI 10.1038/sj.embor.embor730
PII embor730
PMID 12612613
PMC PMC1315828
MeSH Animals Bacterial Proteins / genetics Bacterial Proteins / metabolism Drosophila melanogaster / immunology Drosophila melanogaster / microbiology Drosophila melanogaster / physiology* Host-Parasite Interactions / genetics* Host-Parasite Interactions / physiology Larva / immunology Larva / microbiology Larva / physiology Molecular Sequence Data Pectobacterium carotovorum / genetics* Pectobacterium carotovorum / pathogenicity Pectobacterium carotovorum / physiology
IF 8.383
Times Cited 62
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Drosophila