RRC ID 30704
Author Ueta M, Wada C, Daifuku T, Sako Y, Bessho Y, Kitamura A, Ohniwa RL, Morikawa K, Yoshida H, Kato T, Miyata T, Namba K, Wada A.
Title Conservation of two distinct types of 100S ribosome in bacteria.
Journal Genes Cells
Abstract In bacteria, 70S ribosomes (consisting of 30S and 50S subunits) dimerize to form 100S ribosomes, which were first discovered in Escherichia coli. Ribosome modulation factor (RMF) and hibernation promoting factor (HPF) mediate this dimerization in stationary phase. The 100S ribosome is translationally inactive, but it dissociates into two translationally active 70S ribosomes after transfer from starvation to fresh medium. Therefore, the 100S ribosome is called the 'hibernating ribosome'. The gene encoding RMF is found widely throughout the Gammaproteobacteria class, but is not present in any other bacteria. In this study, 100S ribosome formation in six species of Gammaproteobacteria and eight species belonging to other bacterial classes was compared. There were several marked differences between the two groups: (i) Formation of 100S ribosomes was mediated by RMF and short HPF in Gammaproteobacteria species, similar to E. coli, whereas it was mediated only by long HPF in the other bacterial species; (ii) RMF/short HPF-mediated 100S ribosome formation occurred specifically in stationary phase, whereas long HPF-mediated 100S ribosome formation occurred in all growth phases; and (iii) 100S ribosomes formed by long HPF were much more stable than those formed by RMF and short HPF.
Volume 18(7)
Pages 554-74
Published 2013-7
DOI 10.1111/gtc.12057
PMID 23663662
MeSH Bacteria / chemistry* Bacteria / metabolism Evolution, Molecular* Ribosomal Proteins / analysis Ribosomal Proteins / chemistry Ribosomal Proteins / metabolism Ribosomes / chemistry* Ribosomes / classification* Ribosomes / metabolism
IF 2.048
Times Cited 18
WOS Category GENETICS & HEREDITY CELL BIOLOGY
Resource
DNA material Thermus thermophilus HB8 gene disruption plasmid TTHA1294: TDs07A03 (THR022803)