論文 - 詳細
| RRC ID | 30727 |
|---|---|
| 著者 | Matsumura K, Tanaka T, Kawashima H, Nakatani T. |
| タイトル | Involvement of the estrogen receptor beta in genistein-induced expression of p21(waf1/cip1) in PC-3 prostate cancer cells. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:Dietary genistein, a phytoestrogen derived from soybean, has been suggested as a chemopreventive agent for prostate cancer. Genistein has been reported to exert its anticancer effects via a variety of functional pathways, but the upstream signaling of molecules regulated by genistein remains unclear. In this study, estrogen receptor (ER) beta involvement in genistein-induced expression of cell cycle inhibitors in PC-3 prostate cancer cells was investigated. MATERIALS AND METHODS:The proliferation of PC-3 cells exposed to genistein was measured by the water-soluble tetrazolium salt (WST-1) proliferation assay. The expression of p21, p27 and ERbeta in the PC-3 cells was assessed by quantitative real-time reverse transcription-PCR. ERbeta silencing was performed using a small interfering RNA (siRNA). The transcriptional activity of the p21 promoter was determined by the luciferase reporter assay. RESULTS:Genistein caused marked inhibition of proliferative activity and induced the expression of p21 and ERbeta in the PC-3 cells. The siRNA against ERbeta suppressed the genistein-induced expression of p21 and reduced the transactivation activity of the p21 promoter induced by genistein. CONCLUSION:ERbeta is involved in genistein-induced expression of p21 in PC-3 cells. |
| 巻・号 | 28(2A) |
| ページ | 709-14 |
| 公開日 | 2008-1-1 |
| PMID | 18507011 |
| MeSH | Cell Proliferation / drug effects Cyclin-Dependent Kinase Inhibitor p21 / genetics* Estrogen Receptor beta / physiology* Gene Expression Genistein / pharmacology* Humans Male Prostatic Neoplasms / genetics* RNA, Small Interfering Tumor Cells, Cultured |
| IF | 1.994 |
| 引用数 | 28 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| 遺伝子材料 | pKM2L-phP21 (RDB05893). |