論文 - 詳細
| RRC ID | 30770 |
|---|---|
| 著者 | Yua QB, Ma Q, Kong MM, Zhao TT, Zhang XL, Zhou Q, Huang C, Chong K, Yang ZN. |
| タイトル | AtECB1/MRL7, a thioredoxin-like fold protein with disulfide reductase activity, regulates chloroplast gene expression and chloroplast biogenesis in Arabidopsis thaliana. |
| ジャーナル | Mol Plant |
| Abstract |
Plastid-encoded RNA polymerase (PEP) is closely associated with numerous factors to form PEP complex for plastid gene expression and chloroplast development. However, it is not clear how PEP complex are regulated in chloroplast. Here, one thioredoxin-like fold protein, Arabidopsis early chloroplast biogenesis 1 (AtECB1), an allele of MRL7, was identified to regulate PEP function and chloroplast biogenesis. The knockout lines for AtECB1 displayed albino phenotype and impaired chloroplast development. The transcripts of PEP-dependent plastid genes were barely detected, suggesting that the PEP activity is almost lost in atecb1-1. Although AtECB1 was not identified in PEP complex, a yeast two-hybrid assay and pull-down experiments demonstrated that it can interact with Trx Z and FSD3, two intrinsic subunits of PEP complex, respectively. This indicates that AtECB1 may play a regulatory role for PEP-dependent plastid gene expression through these two subunits. AtECB1 contains a βαβαββα structure in the thioredoxin-like fold domain and lacks the typical C-X-X-C active site motif. Insulin assay demonstrated that AtECB1 harbors disulfide reductase activity in vitro using the purified recombinant AtECB1 protein. This showed that this thioredoxin-like fold protein, AtECB1 also has the thioredoxin activity. AtECB1 may play a role in thioredoxin signaling to regulate plastid gene expression and chloroplast development. |
| 巻・号 | 7(1) |
| ページ | 206-17 |
| 公開日 | 2014-1-1 |
| DOI | 10.1093/mp/sst092 |
| PII | S1674-2052(14)60864-9 |
| PMID | 23956074 |
| MeSH | Amino Acid Sequence Arabidopsis / cytology* Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Chloroplast Thioredoxins / metabolism Chloroplasts / enzymology Chloroplasts / genetics Chloroplasts / metabolism* DNA-Directed RNA Polymerases / metabolism Gene Expression Regulation, Plant* Gene Knockout Techniques Molecular Sequence Data NADH, NADPH Oxidoreductases / metabolism* Protein Transport Thioredoxins / chemistry* Thioredoxins / genetics Thioredoxins / metabolism |
| IF | 12.084 |
| 引用数 | 23 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Peer-review(Publons) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst03066 |