RRC ID 30801
著者 Sato K, Minai Y, Watanabe H.
タイトル Effect of monochromatic visible light on intracellular superoxide anion production and mitochondrial membrane potential of B16F1 and B16F10 murine melanoma cells.
ジャーナル Cell Biol Int
Abstract We have investigated the effect of visible light on animal cells using light-emitting diodes to emit monochromatic visible light (red, yellow, green and blue light). To explore the relevant mechanism of apoptosis, we assessed the intracellular superoxide anion production and mitochondrial membrane potential (ΔΨm) of B16F1 and B16F10 murine melanoma cells after monochromatic light irradiation. Blue light caused ΔΨm depolarization subsequent to elevation of intracellular superoxide production. However, red and yellow light had no affect on both cell lines. Green light induced ΔΨm collapse only in B16F1 melanoma cells. ΔΨm is a key indicator of mitochondrial function, therefore its disruption causes mitochondria-dependent apoptosis. Thus, blue light causes mitochondrial dysfunction and subsequent cell death.
巻・号 37(6)
ページ 633-7
公開日 2013-6-1
DOI 10.1002/cbin.10069
PMID 23404540
MeSH Animals Cell Line, Tumor Light* Melanoma, Experimental Membrane Potential, Mitochondrial / physiology* Membrane Potential, Mitochondrial / radiation effects Mice Mitochondria / physiology Mitochondria / radiation effects Superoxides / metabolism*
IF 2.571
引用数 3
リソース情報
ヒト・動物細胞 B16F10(RCB2630) B16F1(RCB2649)