RRC ID 30813
Author Ayano M, Kani T, Kojima M, Sakakibara H, Kitaoka T, Kuroha T, Angeles-Shim RB, Kitano H, Nagai K, Ashikari M.
Title Gibberellin biosynthesis and signal transduction is essential for internode elongation in deepwater rice.
Journal Plant Cell Environ.
Abstract Under flooded conditions, the leaves and internodes of deepwater rice can elongate above the water surface to capture oxygen and prevent drowning. Our previous studies showed that three major quantitative trait loci (QTL) regulate deepwater-dependent internode elongation in deepwater rice. In this study, we investigated the age-dependent internode elongation in deepwater rice. We also investigated the relationship between deepwater-dependent internode elongation and the phytohormone gibberellin (GA) by physiological and genetic approach using a QTL pyramiding line (NIL-1 + 3 + 12). Deepwater rice did not show internode elongation before the sixth leaf stage under deepwater condition. Additionally, deepwater-dependent internode elongation occurred on the sixth and seventh internodes during the sixth leaf stage. These results indicate that deepwater rice could not start internode elongation until the sixth leaf stage. Ultra-performance liquid chromatography tandem mass-spectrometry (UPLC-MS/MS) method for the phytohormone contents showed a deepwater-dependent GA1 and GA4 accumulation in deepwater rice. Additionally, a GA inhibitor abolished deepwater-dependent internode elongation in deepwater rice. On the contrary, GA feeding mimicked internode elongation under ordinary growth conditions. However, mutations in GA biosynthesis and signal transduction genes blocked deepwater-dependent internode elongation. These data suggested that GA biosynthesis and signal transduction are essential for deepwater-dependent internode elongation in deepwater rice.
Volume 37(10)
Pages 2313-24
Published 2014-10
DOI 10.1111/pce.12377
PMID 24891164
PMC PMC4282320
MeSH Chromatography, High Pressure Liquid Gene Expression Regulation, Plant* Gibberellins / analysis Gibberellins / metabolism* Oryza / drug effects Oryza / genetics Oryza / growth & development Oryza / physiology* Oxygen / metabolism* Plant Growth Regulators / analysis Plant Growth Regulators / metabolism* Plant Leaves / genetics Plant Leaves / growth & development Plant Leaves / physiology Plant Proteins / metabolism Quantitative Trait Loci / genetics Signal Transduction* Tandem Mass Spectrometry Triazoles / pharmacology Water / physiology
IF 5.415
Times Cited 14
Rice C9285