RRC ID 30828
Author Oguma Y, Sawa M, Matsubayashi H, Nonaka M, Tomaru M.
Title P element-induced mutations in Drosophila melanogaster showing high interspecific crossability between D. melanogaster females and D. simulans males.
Journal Jpn. J. Genet.
Abstract Homozygous stocks for the second or the third chromosome of Drosophila melanogaster with a single insertional plwB element were screened for high crossability with D. simulans. Reciprocal crosses between each of these stocks and D. simulans were made, and the insemination rate at two or three days was examined. From two cycles of screening of the original 575 stocks, one stock (# 687) which showed high insemination rate was selected and was backcrossed to a w strain to substitute the background. We obtained a stock which showed 10% insemination rate with D. simulans males (control was 0%). No stocks exhibiting a high crossability with D. simulans females were acquired. Revertant strains, from which the P element had been lost, were obtained from the backcrossed # 687 stock. The insemination rates of 13 revertants to D. simulans males ranged from 1% to 33%. Seven of these 13 were not significantly different from the control line but were significantly different from the backcrossed # 687 stock. It was concluded that the mutation showing high crossability with D. simulans males was caused by the P element transposition.
Volume 70(2)
Pages 197-209
Published 1995-4
PMID 7605672
MeSH Animals Brain / enzymology Crosses, Genetic* DNA Probes DNA Transposable Elements / genetics* Drosophila / genetics Drosophila melanogaster / genetics* Female Gene Expression Regulation, Enzymologic Genes, Insect Genes, Reporter In Situ Hybridization Insemination Male Mutagenesis* Ovary / enzymology Species Specificity Transformation, Genetic beta-Galactosidase / genetics
Times Cited 4