| RRC ID |
30837
|
| 著者 |
Hirai K, Toyohira S, Ohsako T, Yamamoto MT.
|
| タイトル |
Isolation and cytogenetic characterization of male meiotic mutants of Drosophila melanogaster.
|
| ジャーナル |
Genetics
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| Abstract |
Proper segregation of homologous chromosomes in meiosis I is ensured by pairing of homologs and maintenance of sister chromatid cohesion. In male Drosophila melanogaster, meiosis is achiasmatic and homologs pair at limited chromosome regions called pairing sites. We screened for male meiotic mutants to identify genes required for normal pairing and disjunction of homologs. Nondisjunction of the sex and the fourth chromosomes in male meiosis was scored as a mutant phenotype. We screened 2306 mutagenized and 226 natural population-derived second and third chromosomes and obtained seven mutants representing different loci on the second chromosome and one on the third. Five mutants showed relatively mild effects (<10% nondisjunction). mei(2)yh149 and mei(2)yoh7134 affected both the sex and the fourth chromosomes, mei(2)yh217 produced possible sex chromosome-specific nondisjunction, and mei(2)yh15 and mei(2)yh137 produced fourth chromosome-specific nondisjunction. mei(2)yh137 was allelic to the teflon gene required for autosomal pairing. Three mutants exhibited severe defects, producing >10% nondisjunction of the sex and/or the fourth chromosomes. mei(2)ys91 (a new allele of the orientation disruptor gene) and mei(3)M20 induced precocious separation of sister chromatids as early as prometa-phase I. mei(2)yh92 predominantly induced nondisjunction at meiosis I that appeared to be the consequence of failure of the separation of paired homologous chromosomes.
|
| 巻・号 |
166(4)
|
| ページ |
1795-806
|
| 公開日 |
2004-4-1
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| DOI |
10.1534/genetics.166.4.1795
|
| PII |
166/4/1795
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| PMID |
15126399
|
| PMC |
PMC1470820
|
| MeSH |
Animals
Chromosome Segregation / genetics*
Chromosomes / genetics*
Cytogenetic Analysis
DNA Primers
Drosophila melanogaster / genetics*
Ethyl Methanesulfonate
Male
Meiosis / genetics*
Mutation / genetics*
Nondisjunction, Genetic / genetics*
Polymerase Chain Reaction
|
| IF |
4.015
|
| 引用数 |
7
|
|
WOS 分野
|
GENETICS & HEREDITY
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| リソース情報 |
| ショウジョウバエ |
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