論文 - 詳細
| RRC ID | 3087 |
|---|---|
| 著者 | Yonekura-Sakakibara K, Tohge T, Niida R, Saito K. |
| タイトル | Identification of a flavonol 7-O-rhamnosyltransferase gene determining flavonoid pattern in Arabidopsis by transcriptome coexpression analysis and reverse genetics. |
| ジャーナル | J Biol Chem |
| Abstract |
Glycosylation plays a major role in the remarkable chemical diversity of flavonoids in plants including Arabidopsis thaliana. The wide diversity encoded by the large family-1 glycosyltransferase (UGT) gene family makes it difficult to determine the biochemical function of each gene solely from its primary sequence. Here we used transcriptome coexpression analysis combined with a reverse genetics approach to identify a gene that is prominent in determining the flavonoid composition of Arabidopsis. Using transcriptome coexpression analysis accessible on the ATTED-II public data base, the expression pattern of a UGT gene, UGT89C1, was found to be highly correlated with known flavonoid biosynthetic genes. No C-7 rhamnosylated flavonols were detected in either of two T-DNA ugt89c1 mutants. This specific metabolite deficiency in the mutants was complemented by stable transformation with the genomic fragment containing intact UGT89C1. Glutathione S-transferasefused recombinant UGT89C1 protein converted kaempferol 3-O-glucoside to kaempferol 3-O-glucoside-7-O-rhamnoside and recognized 3-O-glycosylated flavonols and UDP-rhamnose as substrates, but not flavonol aglycones, 3-O-glycosylated anthocyanins or other UDP-sugars. These results show that UGT89C1 is a flavonol 7-O-rhamnosyltransferase. The abundance of UGT89C1 transcripts in floral buds was consistent with the flavonoid accumulation of C-7 rhamnosylated flavonols in Arabidopsis organs. Our present study demonstrates that the integration of transcriptome coexpression analysis with a reverse genetic approach is a versatile tool for understanding a multigene family of a metabolic pathway in Arabidopsis. |
| 巻・号 | 282(20) |
| ページ | 14932-41 |
| 公開日 | 2007-5-18 |
| DOI | 10.1074/jbc.M611498200 |
| PII | S0021-9258(20)63648-1 |
| PMID | 17314094 |
| MeSH | Anthocyanins / metabolism Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis Proteins / biosynthesis Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Flavonols / metabolism Gene Expression Gene Expression Regulation, Enzymologic / physiology* Gene Expression Regulation, Plant / physiology* Hexosyltransferases / biosynthesis Hexosyltransferases / genetics* Hexosyltransferases / metabolism Kaempferols / biosynthesis Transcription, Genetic / physiology* |
| IF | 4.238 |
| 引用数 | 181 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda08132 |