| RRC ID |
3087
|
| 著者 |
Yonekura-Sakakibara K, Tohge T, Niida R, Saito K.
|
| タイトル |
Identification of a flavonol 7-O-rhamnosyltransferase gene determining flavonoid pattern in Arabidopsis by transcriptome coexpression analysis and reverse genetics.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Glycosylation plays a major role in the remarkable chemical diversity of flavonoids in plants including Arabidopsis thaliana. The wide diversity encoded by the large family-1 glycosyltransferase (UGT) gene family makes it difficult to determine the biochemical function of each gene solely from its primary sequence. Here we used transcriptome coexpression analysis combined with a reverse genetics approach to identify a gene that is prominent in determining the flavonoid composition of Arabidopsis. Using transcriptome coexpression analysis accessible on the ATTED-II public data base, the expression pattern of a UGT gene, UGT89C1, was found to be highly correlated with known flavonoid biosynthetic genes. No C-7 rhamnosylated flavonols were detected in either of two T-DNA ugt89c1 mutants. This specific metabolite deficiency in the mutants was complemented by stable transformation with the genomic fragment containing intact UGT89C1. Glutathione S-transferasefused recombinant UGT89C1 protein converted kaempferol 3-O-glucoside to kaempferol 3-O-glucoside-7-O-rhamnoside and recognized 3-O-glycosylated flavonols and UDP-rhamnose as substrates, but not flavonol aglycones, 3-O-glycosylated anthocyanins or other UDP-sugars. These results show that UGT89C1 is a flavonol 7-O-rhamnosyltransferase. The abundance of UGT89C1 transcripts in floral buds was consistent with the flavonoid accumulation of C-7 rhamnosylated flavonols in Arabidopsis organs. Our present study demonstrates that the integration of transcriptome coexpression analysis with a reverse genetic approach is a versatile tool for understanding a multigene family of a metabolic pathway in Arabidopsis.
|
| 巻・号 |
282(20)
|
| ページ |
14932-41
|
| 公開日 |
2007-5-18
|
| DOI |
10.1074/jbc.M611498200
|
| PII |
S0021-9258(20)63648-1
|
| PMID |
17314094
|
| MeSH |
Anthocyanins / metabolism
Arabidopsis / enzymology
Arabidopsis / genetics*
Arabidopsis Proteins / biosynthesis
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Flavonols / metabolism
Gene Expression
Gene Expression Regulation, Enzymologic / physiology*
Gene Expression Regulation, Plant / physiology*
Hexosyltransferases / biosynthesis
Hexosyltransferases / genetics*
Hexosyltransferases / metabolism
Kaempferols / biosynthesis
Transcription, Genetic / physiology*
|
| IF |
4.238
|
| 引用数 |
181
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda08132 |
