RRC ID 30899
Author Shibahara S, Tomita Y, Tagami H, Müller RM, Cohen T.
Title Molecular basis for the heterogeneity of human tyrosinase.
Journal Tohoku J Exp Med
Abstract A cDNA clone, pHT gamma 1, representing human tyrosinase mRNA was isolated by screening a melanoma cDNA library with a synthetic oligonucleotide complementary to a segment of the human tyrosinase cDNA, Pmel 34 [Kwon et al. (1987) Proc. nat. Acad. Sci. USA 84, 7473-7477]. However, there are a number of differences in the nucleotide sequence between two cDNAs, pHT gamma 1 and Pmel 34, particularly in the region coding for the carboxyl terminus of the enzyme (putative exon 5). We therefore cloned the genomic DNA segment carrying the exon 5 of the human tyrosinase gene by screening a human placental genomic DNA library with a cloned cDNA probe. The nucleotide sequences of human tyrosinase cDNA as well as part of its gene were determined. Mature human tyrosinase is composed of 511 amino acids with a molecular weight of 58,000. We provide evidence for the presence of at least two species of human tyrosinase mRNA generated by alternative splicing in human pigmented melanoma cells.
Volume 156(4)
Pages 403-14
Published 1988-12-1
DOI 10.1620/tjem.156.403
PMID 2854305
MeSH Amino Acid Sequence Base Sequence Catechol Oxidase / genetics* Cloning, Molecular DNA / isolation & purification DNA, Recombinant / analysis Endonucleases Humans Melanoma / analysis Melanoma / pathology Molecular Sequence Data Monophenol Monooxygenase / analysis Monophenol Monooxygenase / genetics* RNA, Messenger / analysis Restriction Mapping Sequence Homology, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases Tumor Cells, Cultured / analysis
IF 1.441
Times Cited 90
DNA material pHT gamma 1 (RDB01205) pRHOHT2 (RDB02082)