論文 - 詳細
| RRC ID | 30899 |
|---|---|
| 著者 | Shibahara S, Tomita Y, Tagami H, Müller RM, Cohen T. |
| タイトル | Molecular basis for the heterogeneity of human tyrosinase. |
| ジャーナル | Tohoku J Exp Med |
| Abstract |
A cDNA clone, pHT gamma 1, representing human tyrosinase mRNA was isolated by screening a melanoma cDNA library with a synthetic oligonucleotide complementary to a segment of the human tyrosinase cDNA, Pmel 34 [Kwon et al. (1987) Proc. nat. Acad. Sci. USA 84, 7473-7477]. However, there are a number of differences in the nucleotide sequence between two cDNAs, pHT gamma 1 and Pmel 34, particularly in the region coding for the carboxyl terminus of the enzyme (putative exon 5). We therefore cloned the genomic DNA segment carrying the exon 5 of the human tyrosinase gene by screening a human placental genomic DNA library with a cloned cDNA probe. The nucleotide sequences of human tyrosinase cDNA as well as part of its gene were determined. Mature human tyrosinase is composed of 511 amino acids with a molecular weight of 58,000. We provide evidence for the presence of at least two species of human tyrosinase mRNA generated by alternative splicing in human pigmented melanoma cells. |
| 巻・号 | 156(4) |
| ページ | 403-14 |
| 公開日 | 1988-12-1 |
| DOI | 10.1620/tjem.156.403 |
| PMID | 2854305 |
| MeSH | Amino Acid Sequence Base Sequence Catechol Oxidase / genetics* Cloning, Molecular DNA / isolation & purification DNA, Recombinant / analysis Endonucleases Humans Melanoma / analysis Melanoma / pathology Molecular Sequence Data Monophenol Monooxygenase / analysis Monophenol Monooxygenase / genetics* RNA, Messenger / analysis Restriction Mapping Sequence Homology, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases Tumor Cells, Cultured / analysis |
| IF | 1.441 |
| 引用数 | 90 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pHT gamma 1 (RDB01205) pRHOHT2 (RDB02082) |