| RRC ID |
30941
|
| Author |
Ishii J, Kondo T, Makino H, Ogura A, Matsuda F, Kondo A.
|
| Title |
Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae.
|
| Journal |
FEMS Yeast Res
|
| Abstract |
Yeast has the potential to be used in bulk-scale fermentative production of fuels and chemicals due to its tolerance for low pH and robustness for autolysis. However, expression of multiple external genes in one host yeast strain is considerably labor-intensive due to the lack of polycistronic transcription. To promote the metabolic engineering of yeast, we generated systematic and convenient genetic engineering tools to express multiple genes in Saccharomyces cerevisiae. We constructed a series of multi-copy and integration vector sets for concurrently expressing two or three genes in S. cerevisiae by embedding three classical promoters. The comparative expression capabilities of the constructed vectors were monitored with green fluorescent protein, and the concurrent expression of genes was monitored with three different fluorescent proteins. Our multiple gene expression tool will be helpful to the advanced construction of genetically engineered yeast strains in a variety of research fields other than metabolic engineering.
|
| Volume |
14(3)
|
| Pages |
399-411
|
| Published |
2014-5-1
|
| DOI |
10.1111/1567-1364.12138
|
| PMID |
24447461
|
| MeSH |
Gene Expression*
Genes, Reporter
Genetic Vectors*
Genetics, Microbial / methods*
Luminescent Proteins / analysis
Luminescent Proteins / genetics
Metabolic Engineering / methods*
Molecular Biology / methods*
Promoter Regions, Genetic
Recombinant Proteins / analysis
Recombinant Proteins / genetics
Saccharomyces cerevisiae / genetics*
|
| IF |
3.193
|
| Times Cited |
24
|
|
WOS Category
|
MYCOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| Resource |
| Yeast |
BYP7579
BYP7580
BYP7581
BYP7582
BYP7583
BYP7584
BYP7585
BYP7586
BYP7587
BYP7588 |
