Reference - Detail
| RRC ID | 30941 |
|---|---|
| Author | Ishii J, Kondo T, Makino H, Ogura A, Matsuda F, Kondo A. |
| Title | Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae. |
| Journal | FEMS Yeast Res |
| Abstract |
Yeast has the potential to be used in bulk-scale fermentative production of fuels and chemicals due to its tolerance for low pH and robustness for autolysis. However, expression of multiple external genes in one host yeast strain is considerably labor-intensive due to the lack of polycistronic transcription. To promote the metabolic engineering of yeast, we generated systematic and convenient genetic engineering tools to express multiple genes in Saccharomyces cerevisiae. We constructed a series of multi-copy and integration vector sets for concurrently expressing two or three genes in S. cerevisiae by embedding three classical promoters. The comparative expression capabilities of the constructed vectors were monitored with green fluorescent protein, and the concurrent expression of genes was monitored with three different fluorescent proteins. Our multiple gene expression tool will be helpful to the advanced construction of genetically engineered yeast strains in a variety of research fields other than metabolic engineering. |
| Volume | 14(3) |
| Pages | 399-411 |
| Published | 2014-5-1 |
| DOI | 10.1111/1567-1364.12138 |
| PMID | 24447461 |
| MeSH | Gene Expression* Genes, Reporter Genetic Vectors* Genetics, Microbial / methods* Luminescent Proteins / analysis Luminescent Proteins / genetics Metabolic Engineering / methods* Molecular Biology / methods* Promoter Regions, Genetic Recombinant Proteins / analysis Recombinant Proteins / genetics Saccharomyces cerevisiae / genetics* |
| IF | 3.193 |
| Times Cited | 24 |
| WOS Category | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | BYP7579 BYP7580 BYP7581 BYP7582 BYP7583 BYP7584 BYP7585 BYP7586 BYP7587 BYP7588 |