RRC ID 30959
Author Yamashino T, Yamawaki S, Hagui E, Ishida K, Ueoka-Nakanishi H, Nakamichi N, Mizuno T.
Title Clock-controlled and FLOWERING LOCUS T (FT)-dependent photoperiodic pathway in Lotus japonicus II: characterization of a microRNA implicated in the control of flowering time.
Journal Biosci. Biotechnol. Biochem.
Abstract Plant circadian clock generates rhythms with a period close to 24 h, and it controls a wide variety of physiological and developmental events, including the transition to reproductive growth (or flowering). During the last decade, significant research progress in Arabidopsis thaliana has been made in defining the molecular mechanism by which the circadian clock regulates flowering time in response to changes in photoperiod. In Lotus japonicus, we have found that LjFTa, which encodes a ortholog of the Arabidopsis FLOWERING LOCUS T (FT), plays an important role in the promotion of flowering, but it is not clear how the expression of LjFTa is regulated in L. japonicus. Based on current knowledge of photoperiodic control of flowering time in A. thaliana, here we examined whether a microRNA is involved in the activation of LjFTa in L. japonicus. Two putative L. japonicus genes that are responsible for the production of miR172 (designated LjmiR172a and LjmiR172b) were cloned. Overexpression of LjmiR172a/b in A. thaliana resulted in markedly accelerated flowering through enhancement of the expression of FT, concomitantly reducing the expression level of TARGET OF EARLY ACTIVATION TAGGED 1 (TOE1) transcripts, the protein product of which functions as a transcriptional repressor of FT. These results suggest that LjmiR172 genes play a positive role in the LjFTa-mediated promotion of flowering in L. japonicus.
Volume 77(6)
Pages 1179-85
Published 2013
DOI 10.1271/bbb.120872
PII DN/JST.JSTAGE/bbb/120872
PMID 23748785
MeSH Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis Proteins / genetics Circadian Clocks / genetics Circadian Rhythm / genetics DNA-Binding Proteins / genetics Flowers / genetics Flowers / growth & development* Gene Expression Regulation, Plant Loteae / genetics Loteae / growth & development* MicroRNAs / genetics* MicroRNAs / metabolism Photoperiod* Sequence Homology, Amino Acid
IF 1.255
Times Cited 4
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Lotus / Glycine Miyakojima MG-20