RRC ID 30967
Author Sugiyama A, Linley PJ, Sasaki K, Kumano T, Yamamoto H, Shitan N, Ohara K, Takanashi K, Harada E, Hasegawa H, Terakawa T, Kuzuyama T, Yazaki K.
Title Metabolic engineering for the production of prenylated polyphenols in transgenic legume plants using bacterial and plant prenyltransferases.
Journal Metab. Eng.
Abstract Prenylated polyphenols are secondary metabolites beneficial for human health because of their various biological activities. Metabolic engineering was performed using Streptomyces and Sophora flavescens prenyltransferase genes to produce prenylated polyphenols in transgenic legume plants. Three Streptomyces genes, NphB, SCO7190, and NovQ, whose gene products have broad substrate specificity, were overexpressed in a model legume, Lotus japonicus, in the cytosol, plastids or mitochondria with modification to induce the protein localization. Two plant genes, N8DT and G6DT, from Sophora flavescens whose gene products show narrow substrate specificity were also overexpressed in Lotus japonicus. Prenylated polyphenols were undetectable in these plants; however, supplementation of a flavonoid substrate resulted in the production of prenylated polyphenols such as 7-O-geranylgenistein, 6-dimethylallylnaringenin, 6-dimethylallylgenistein, 8-dimethylallynaringenin, and 6-dimethylallylgenistein in transgenic plants. Although transformants with the native NovQ did not produce prenylated polyphenols, modification of its codon usage led to the production of 6-dimethylallylnaringenin and 6-dimethylallylgenistein in transformants following naringenin supplementation. Prenylated polyphenols were not produced in mitochondrial-targeted transformants even under substrate feeding. SCO7190 was also expressed in soybean, and dimethylallylapigenin and dimethylallyldaidzein were produced by supplementing naringenin. This study demonstrated the potential for the production of novel prenylated polyphenols in transgenic plants. In particular, the enzymatic properties of prenyltransferases seemed to be altered in transgenic plants in a host species-dependent manner.
Volume 13(6)
Pages 629-37
Published 2011-11
DOI 10.1016/j.ymben.2011.07.003
PII S1096-7176(11)00077-2
PMID 21835257
MeSH Dimethylallyltranstransferase / genetics Dimethylallyltranstransferase / metabolism* Flavanones / administration & dosage Loteae / enzymology* Loteae / genetics Metabolic Engineering / methods* Plants, Genetically Modified / enzymology* Plants, Genetically Modified / genetics Polyphenols / biosynthesis* Prenylation / genetics Sophora / enzymology Sophora / genetics Soybeans / enzymology* Soybeans / genetics Streptomyces / enzymology Streptomyces / genetics Substrate Specificity
IF 7.674
Times Cited 21
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Lotus / Glycine Gifu B-129 Miyakojima MG-20