論文 - 詳細
| RRC ID | 30990 |
|---|---|
| 著者 | Takanashi K, Yokosho K, Saeki K, Sugiyama A, Sato S, Tabata S, Ma JF, Yazaki K. |
| タイトル | LjMATE1: a citrate transporter responsible for iron supply to the nodule infection zone of Lotus japonicus. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
Symbiotic nitrogen fixation by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. While exchanged molecules imply nitrogen compounds, carbohydrates and also various minerals, knowledge of the molecular basis of plant transporters that mediate those metabolite exchanges is still limited. In this study, we have shown that a multidrug and toxic compound extrusion (MATE) protein, LjMATE1, is specifically induced during nodule formation, which nearly paralleled nodule maturation, in a model legume Lotus japonicus. Reporter gene experiments indicated that the expression of LjMATE1 was restricted to the infection zone of nodules. To characterize the transport function of LjMATE1, we conducted a biochemical analysis using a heterologous expression system, Xenopus oocytes, and found that LjMATE1 is a specific transporter for citrate. The physiological role of LjMATE1 was analyzed after generation of L. japonicus RNA interference (RNAi) lines. One RNAi knock-down line revealed limited growth under nitrogen-deficient conditions with inoculation of rhizobia compared with the controls (the wild type and an RNAi line in which LjMATE1 was not suppressed). It was noteworthy that Fe localization was clearly altered in nodule tissues of the knock-down line. These results strongly suggest that LjMATE1 is a nodule-specific transporter that assists the translocation of Fe from the root to nodules by providing citrate. |
| 巻・号 | 54(4) |
| ページ | 585-94 |
| 公開日 | 2013-4-1 |
| DOI | 10.1093/pcp/pct019 |
| PII | pct019 |
| PMID | 23385147 |
| MeSH | Carrier Proteins / genetics Carrier Proteins / metabolism* Iron / metabolism* Lotus / genetics Lotus / metabolism* Lotus / microbiology Molecular Sequence Data Plant Proteins / genetics Plant Proteins / metabolism Plant Roots / genetics Plant Roots / metabolism Plant Roots / microbiology Root Nodules, Plant / genetics Root Nodules, Plant / metabolism* Root Nodules, Plant / microbiology |
| IF | 4.062 |
| 引用数 | 42 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | Miyakojima MG-20 pUB-GWS-GFP/Hyg |