| RRC ID |
31001
|
| Author |
Chungopast S, Thapanapongworakul P, Matsuura H, Van Dao T, Asahi T, Tada K, Tajima S, Nomura M.
|
| Title |
Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus.
|
| Journal |
J Plant Physiol
|
| Abstract |
In this study, we focused on the effect of glutamine synthetase (GSI) activity in Mesorhizobium loti on the symbiosis between the host plant, Lotus japonicus, and the bacteroids. We used a signature-tagged mutant of M. loti (STM30) with a transposon inserted into the GSI (mll0343) gene. The L. japonicus plants inoculated with STM30 had significantly more nodules, and the occurrence of senesced nodules was much higher than in plants inoculated with the wild-type. The acetylene reduction activity (ARA) per nodule inoculated with STM30 was lowered compared to the control. Also, the concentration of chlorophyll, glutamine, and asparagine in leaves of STM30-infected plants was found to be reduced. Taken together, these data demonstrate that a GSI deficiency in M. loti differentially affects legume-rhizobia symbiosis by modifying nodule development and metabolic processes.
|
| Volume |
171(5)
|
| Pages |
104-8
|
| Published |
2014-3-1
|
| DOI |
10.1016/j.jplph.2013.10.015
|
| PII |
S0176-1617(13)00424-0
|
| PMID |
24484964
|
| MeSH |
Glutamate-Ammonia Ligase / deficiency
Glutamate-Ammonia Ligase / genetics*
Lotus / metabolism*
Lotus / microbiology*
Mesorhizobium / genetics
Mesorhizobium / metabolism
Mesorhizobium / physiology*
Nitrogen Fixation*
Plant Roots / metabolism
Plant Roots / microbiology
Real-Time Polymerase Chain Reaction
Symbiosis*
|
| IF |
3.013
|
| Times Cited |
8
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Lotus / Glycine |
STM clone ID
10T05g06 |
