RRC ID 31010
Author Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ.
Title A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum.
Journal Plasmid
Abstract A new set of extrachromosomal Dictyostelium expression vectors is presented that can be modified according to the experimental needs with minimal cloning efforts. To achieve this, the vector consists of four functional regions that are separated by unique restriction sites, (1) an Escherichia coli replication region, and regions for (2) replication, (3) selection and (4) protein expression in Dictyostelium. Each region was trimmed down to its smallest possible size. A basic expression vector can be constructed from these modules with a size of only 6.8 kb. By exchanging modules, a large number of vectors with different properties can be constructed. The resulting set of vectors allows most basic expression needs, such as immuno blotting, protein purification, visualization of protein localization and identification of protein-protein interactions. In addition, two genes can be simultaneously expressed on one vector, which yields far more synchronous levels of expression than when expressing two genes on separate plasmids.
Volume 61(2)
Pages 110-8
Published 2009-3-1
DOI 10.1016/j.plasmid.2008.11.003
PII S0147-619X(08)00105-4
PMID 19063918
MeSH Animals Cloning, Molecular* Dictyostelium / genetics* Escherichia coli / genetics Gene Expression Genetic Vectors / genetics Genetic Vectors / physiology* Plasmids / genetics Plasmids / physiology*
IF 2.805
Times Cited 118
Cellular slime molds G90008 G90009 G90067 G90068 G90069 G90070 G90071 G90072 G90073 G90074 G90075