RRC ID 31011
著者 Patel H, Barber DL.
タイトル A developmentally regulated Na-H exchanger in Dictyostelium discoideum is necessary for cell polarity during chemotaxis.
ジャーナル J Cell Biol
Abstract Increased intracellular H(+) efflux is speculated to be an evolutionarily conserved mechanism necessary for rapid assembly of cytoskeletal filaments and for morphological polarity during cell motility. In Dictyostelium discoideum, increased intracellular pH through undefined transport mechanisms plays a key role in directed cell movement. We report that a developmentally regulated Na-H exchanger in Dictyostelium discoideum (DdNHE1) localizes to the leading edge of polarized cells and is necessary for intracellular pH homeostasis and for efficient chemotaxis. Starved DdNHE1-null cells (Ddnhe1(-)) differentiate, and in response to the chemoattractant cAMP they retain directional sensing; however, they cannot attain a polarized morphology, but instead extend mislocalized pseudopodia around the cell and exhibit decreased velocity. Consistent with impaired polarity, in response to chemoattractant, Ddnhe1(-) cells lack a leading edge localization of F-actin and have significantly attenuated de novo F-actin polymerization but increased abundance of membrane-associated phosphatidylinositol 3,4,5-trisphosphate (PI((3,4,5))P(3)). These findings indicate that during chemotaxis DdNHE1 is necessary for establishing the kinetics of actin polymerization and PI((3,4,5))P(3) production and for attaining a polarized phenotype.
巻・号 169(2)
ページ 321-9
公開日 2005-4-25
DOI 10.1083/jcb.200412145
PII jcb.200412145
PMID 15851518
PMC PMC2171856
MeSH Actins / metabolism Animals Chemotaxis / physiology* Dictyostelium / physiology* Homeostasis / genetics Homeostasis / physiology Hydrogen-Ion Concentration Phosphatidylinositol Phosphates / metabolism Protozoan Proteins / genetics Protozoan Proteins / metabolism* Pseudopodia / genetics Pseudopodia / physiology* Sodium-Hydrogen Exchangers / genetics Sodium-Hydrogen Exchangers / metabolism*
IF 8.811
引用数 52
WOS 分野 CELL BIOLOGY
リソース情報
細胞性粘菌 S90421 S90422