Reference - Detail
RRC ID | 31264 |
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Author | Yamano T, Iguchi H, Fukuzawa H. |
Title | Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal. |
Journal | J Biosci Bioeng |
Abstract |
Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions. |
Volume | 115(6) |
Pages | 691-4 |
Published | 2013-6-1 |
DOI | 10.1016/j.jbiosc.2012.12.020 |
PII | S1389-1723(12)00534-8 |
PMID | 23333644 |
MeSH | Cell Wall Chlamydomonas reinhardtii / genetics* Electroporation / methods* Transformation, Genetic* |
IF | 2.366 |
Times Cited | 59 |
WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
Resource | |
Algae | NIES-2235 |