Reference - Detail
| RRC ID | 31264 |
|---|---|
| Author | Yamano T, Iguchi H, Fukuzawa H. |
| Title | Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal. |
| Journal | J Biosci Bioeng |
| Abstract |
Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions. |
| Volume | 115(6) |
| Pages | 691-4 |
| Published | 2013-6-1 |
| DOI | 10.1016/j.jbiosc.2012.12.020 |
| PII | S1389-1723(12)00534-8 |
| PMID | 23333644 |
| MeSH | Cell Wall Chlamydomonas reinhardtii / genetics* Electroporation / methods* Transformation, Genetic* |
| IF | 2.366 |
| Times Cited | 59 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Resource | |
| Algae | NIES-2235 |